ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Molecular origins of the first imported dengue fever case at frontier port in Qinhuangdao
Received date: 2014-10-23
Online published: 2015-04-20
Supported by
Supported by the Major National Science and Technology Projects for China (No. 2013ZX10004-101-006, 2013ZX10004805-008)
Objective To trace the virus from an imported viral hemorrhagic fever case in Qinhuangdao in 2012, molecular characteristics of the pathogen were studied. Methods The serum collected from the suspected case was detected for IgM antibody by ELISA, and viral nucleic acid of serum and urine samples were detected by real-time RT-PCR. Furthermore, NS1 segment was amplified by RT-PCR and sequenced. The genetic and phylogenetic analysis based on NS1 was computed. Results IgM anti-DENV was found in the serum QHD-01-BS1, BS2, and the NS1 gene sequences of serotype 2 of Dengue virus (DEN2) were recovered from the serum QHD-01-BS1 and the urine QHD-01-US respectively. The comparison showed the strains QHD-01-BS1 and QHD-01-US were very closely related to GWL39 INDI-01, GWL18 INDI-01 the COM genotype)isolated from India, with 97.3% sequence identity and 0.04 genetic distance. The further phylogenetic tree indicated the strains QHD-01-BS1 and QHD-01-US were classified as a lineage with GWL39 INDI-01, GWL18 INDI-01, belonged to genotype COM. Conclusion These results suggest the imported viral hemorrhagic fever case was infected by DENV2 from India and most likely infected in India.
Key words： Imported case; Dengue virus; Molecular origin; Genotype
YANG Peng-fei, YAN Qing-li, ZHANG Li-ping, NIE Wei-zhong, ZHEN Wei, MA Xue-zheng, BAI Hong-yan, LIU Chun-cheng, SHI Yu-jun, CHEN Yue, SUN Xiao-hong, HU Kong-xin . Molecular origins of the first imported dengue fever case at frontier port in Qinhuangdao[J]. Chinese Journal of Vector Biology and Control, 2015 , 26(2) : 155 -158 . DOI: 10.11853/j.issn.1003.4692.2015.02.012
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