ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
MLVA analysis on Yersinia pestis isolated from plague foci in Hebei province, China
Received date: 2014-10-13
Online published: 2015-04-20
Supported by
Supported by the Special Research Program for Health(No. 201202021)and the Medical Science Research Key Project in Hebei Province(No. ZL20140119)
Objective To characterize the genosome of 116 Yersinia pestis strains isolated from plague foci in Hebei province in China. Methods All the strains were detected by polymerase chain reaction (PCR) with 15 pairs variable number tandem repeat (VNTR) provided by China CDC, then the length of results were analyzed. Results The numbers and lengths of the repeat sequences from all strains are same with the single primer, and different with the different primers, such as the length of all stains with the prime M52 is 153 bp, and the number of the repeat sequences is 3, then with the prime M59, the length and the number is 250 bp and 3 respectively. Conclusion Multiple loci VNTR analysis (MLVA) genetic typing is reliable, and it is stable genetic mark of Y. pestis from Hebei province. To build the database of the plague with MLVA is useful for the investigation of the plague variation and source.
Key words： Yersinia pestis; Multiple loci VNTR analysis; Genetic typing
WANG Hai-feng, YANG Shun-lin, ZHOU Song, SHI Xian-ming, HU Le-le, LIU He-zhi, LIANG Ying . MLVA analysis on Yersinia pestis isolated from plague foci in Hebei province, China[J]. Chinese Journal of Vector Biology and Control, 2015 , 26(2) : 141 -144 . DOI: 10.11853/j.issn.1003.4692.2015.02.008
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