ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Rapid detection of Japanese encephalitis virus from Culex tritaeniorhynchus by real-time fluorescence reverse- transcriptase polymerase chain reaction
Received date: 2014-10-15
Online published: 2015-04-20
Objective To rapidly detect Japanese encephalitis virus (JEV) from Culex tritaeniorhynchus in Chengdu by real-time fluorescence reverse-transcriptase polymerase chain reaction (RT-PCR) assay, and to analyze the genotype of JEV. Methods Real-time fluorescence RT-PCR was used for detection of JEV infection. PrM segments of JEV from the nucleonic acid detected were amplified by one step RT-PCR, and were sequenced．Clustal X1.83 and Mega 5.0 were used to construct phylogenetic tree and analyze genotype and homology. Results A total of 10 656 mosquitoes of Cx. tritaeniorhynchus were collected with 219 pools in 2012. Seven pools of Cx. tritaeniorhynchus were positive for JEV at 3.2%. Based on sequence analysis, seven samples were identified to be genotypeⅠ JEV with the 674 bp sequence of PrM. Compared with some sequences of JEV GⅠ, the seven samples showed nucleotide and amino acid homologies of 91.5%-100% and 94.9%-100%, respectively, and were highly homology with the Sichuan strain of JEV (SC09-X08). Conclusion The prevalent JEV from Cx. tritaeniorhynchus in Chengdu was quickly detected by real-time fluorescence RT-PCR assay and confirmed to be genotypeⅠ.
TIAN Wen-jia, DENG Liang-li, LI Peng, ZHANG Wei, LIU Zhu, MA Lin . Rapid detection of Japanese encephalitis virus from Culex tritaeniorhynchus by real-time fluorescence reverse- transcriptase polymerase chain reaction[J]. Chinese Journal of Vector Biology and Control, 2015 , 26(2) : 137 -140 . DOI: 10.11853/j.issn.1003.4692.2015.02.007
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