Surveillance on mosquito-borne arboviruses in Xixian county and Xin'an county, Henan province, 2012

ZHENG Ya-yun, FU Shi-hong, TANG Xiao-yan, LI Xing-le, SHANG Si-yuan, XU Chao, LIANG Guo-dong

doi:10.11853/j.issn.1003.4692.2015.02.005

Chinese Journal of Vector Biology and Control >

2015 , Vol. 26 >Issue 2: 127 - 132

DOI: https://doi.org/10.11853/j.issn.1003.4692.2015.02.005

Surveillance on mosquito-borne arboviruses in Xixian county and Xin'an county, Henan province, 2012

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1 State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
2 Henan Center for Disease Control and Prevention;
3 Xin'an Center for Disease Control and Prevention;
4 Xixian Center for Disease Control and Prevention

Received date: 2014-11-20

Online published: 2015-04-20

Supported by

Supported by the National Natural Science Foundation of China(No. 81290342)and the National Key Basic Research Program of China(973 Program)(No. 2011CB504702)

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Abstract

Objective To understand the species, distribution and genotype of mosquito-borne arboviruses in some regions of Henan province. Methods Mosquito samples were collected from Xixian county and Xin'an county in Henan province from May to August, 2012. After species identification, mosquitoes were inter-cells cross cultured for viral isolation. RT-PCR using specific primer for common arboviruses was used to identify the positive isolates. Molecular biological analysis were conducted by using software of Clustal X2.1, MegAlign, Genedoc 3.2 and Mega v5.1, and genotype for the virus was identified. Results A total of 7149 mosquitoes which belong to 5 species in 4 genera were collected. The predominant mosquito specie in Xin'an county was Armigeres subalbatus (2055, 51.36%), but the mainly species in Xixian county was Culex pipens pallens (2964, 94.16%). Five strains from Cx. pipens pallens and Cx. tritaeniorhynchus were identified as genotypeⅠ Japanese encephalitis virus (JEV), which caused slightly cytopathic effect (CPE) in C6/36 cells, and no CPE in BHK-21. But severe CPE was observed in BHK-21 when 3 days after inoculating the C6/36 culture of the viruses onto BHK-21 cells. Conclusion In Xixian county and Xin'an county of Henan province, b

Key words： Mosquito-borne arboviruses; Japanese encephalitis virus; Genotype

Cite this article

ZHENG Ya-yun, FU Shi-hong, TANG Xiao-yan, LI Xing-le, SHANG Si-yuan, XU Chao, LIANG Guo-dong . Surveillance on mosquito-borne arboviruses in Xixian county and Xin'an county, Henan province, 2012[J]. Chinese Journal of Vector Biology and Control, 2015 , 26(2) : 127 -132 . DOI: 10.11853/j.issn.1003.4692.2015.02.005

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