ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Investigation of infection with Bartonella in domestic and feral cats in Beijing, China
Received date: 2014-09-30
Online published: 2015-02-20
Supported by
Supported by the Major National Science and Technology Subject(No. 2008ZX10004-010)
Objective To investigate the status of infection with Bartonella in domestic and feral cats in Beijing, China. Methods EDTA-anticoagulated blood and serum samples were collected from cats, and the relevant epidemiological information was recorded. The anticoagulated blood was diluted 1:4 in sterilized tryptic soy broth and a 100 μl aliquot was inoculated onto the brain-heart infusion agar supplemented with 5% defibrinated sheep blood followed by culturing in a 5% CO2 incubator at 37 ℃ for 45 days. Bartonella-like isolates from the culture were examined by PCR analysis using the primers for gltA, ftsZ, and ribC. The PCR products were sequenced, and homologous comparison and phylogenetic analysis were performed to identify the species of Bartonella. Indirect immunofluorescence assay was carried out to measure the titer of anti-Bartonella henselae IIFT (IgG) in the serum samples. Data obtained from the experiments and the epidemiological information collected in the field were analyzed by the SPSS 13.0 software. Results The positive rate for Bartonella culture isolates was 13.8% in the cats tested in Beijing, and all the 22 strains obtained were B. henselae. The seropositivity rate for anti-B. henselae IgG was 39.4%. The feral cats, flea-infested cats, and kittens had significantly higher positive rates for Bartonella isolates (30.4%, 36.6%, and 27.9%, respectively). The seropositivity rate for anti-B. henselae IgG in flea-infested cats (61.0%) was also significantly higher than that in uninfested cats (31.9%). Conclusion Bartonella infection is common in both domestic and feral cats in Beijing, and the species is identified to be human pathogen B. henselae. Bartonella infection in humans could be avoided by flea control and preventing flea infestation in domestic cats, and strengthening the control of feral cats.
Key words： Bartonella; Domestic cat; Feral cat; Cat scratch disease
HUANG Ru-ting, SONG Xiu-ping, YANG Xiu-huan, LI Dong-mei, ZHAO Fan, SUN Ji-min, LIU Qi-yong . Investigation of infection with Bartonella in domestic and feral cats in Beijing, China[J]. Chinese Journal of Vector Biology and Control, 2015 , 26(1) : 19 -22 . DOI: 10.11853/j.issn.1003.4692.2015.01.005
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