ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
First detection of Bartonella henselae infection in Haemaphysalis longicornis
Received date: 2014-09-25
Online published: 2015-02-20
Supported by
Supported by the National Natural Science Foundation of China(No. 30600513)and the National Science and Technology Major Project for Infectious Diseases in China(No. 2012ZX10004-219)
Objective To detect the infection with Bartonella henselae in Haemaphysalis longicornis. Methods Haemaphysalis longicornis captured in Lingshou county, Shijiazhuang, Hebei province, China, were divided into groups. The H. longicornis in each group was disinfected, sterilized, and grinded. Half of the grinded mixture was directly used for DNA extraction and polymerase chain reaction (PCR) analysis;the other half was inoculated onto tryptic soy agar supplemented with 5% defibrinated sheep blood followed by culturing in a 5% CO2 incubator at 37 ℃, and the suspected colonies were selected for PCR analysis. The PCR products that produced positive bands were sequenced, and the nucleotide sequences obtained were aligned with known sequences in GenBank. Results A positive band was observed in one sample from direct DNA extraction, the sequence of which was found to belong to B. henselae by alignment analysis. Positive bands were observed in two DNA samples isolated from bacterial cultures, but no results were produced from sequencing. Conclusion Haemaphysalis longicornis collected in Lingshou county, Shijiazhuang, are infected with B. henselae. This is the first detection of B. henselae infection in H. longicornis.
WU Hai-xia, LI Zhi-fang, LIU Qi-yong, ZHANG Wei-dong, LI Dong-mei, MA Huai-lei, LU Liang, LIU Jing-li . First detection of Bartonella henselae infection in Haemaphysalis longicornis[J]. Chinese Journal of Vector Biology and Control, 2015 , 26(1) : 16 -18 . DOI: 10.11853/j.issn.1003.4692.2015.01.004
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