Objective To investigate the effect of knockdown resistance mutations on the deltamethrin resistance of Anopheles sinensis. Methods The deltamethrin resistance of An. sinensis was determined by WHO cones test at six investigation sites in Hefei, China, from 2011 to 2012. Eighty An. sinensis specimens were randomly selected from each of the survival and death populations after the resistance test. DNA was extracted as templates for PCR amplification, and the IIS5-S6 transmembrane segments of voltage?gated sodium channel (VGSC) gene were cloned and sequenced. Chi?square test was used to compare mutation types between the two populations with SPSS software. Results Sequence analysis revealed two types of mutations (TTG to TTT and TTG to TGT) in both groups, with four combinations, i.e., heterozygous mutations TTG(T), TG(T)G(T), and TG(T)T and homozygous mutation (TTT). TGT mutation accounted for 36.9% of the survival population, higher than that of the death population (19.4%) (χ2＝17.571, P＜0.01), according to the comparison of mutation types (TTG, TTT, and TGT) between the two populations. Conclusion TGT mutation is more associated with deltamethrin resistance than TTT mutation.
SONG Er-ping, TAN Qi-gan, GUAN Huai-bin, SONG Xue-song, SONG Er-yong, WU Xiao-chen, YUAN Liang,WANG Yu-jiao, NI Xing, NI Yang-yang, ZHANG Liang-liang, WU Song
. Comparison of knockdown resistance mutations between survival and death populations of Anopheles sinensis after deltamethrin resistance test[J]. Chinese Journal of Vector Biology and Control, 2014
, 25(6)
: 527
-533
.
DOI: 10.11853/j.issn.1003.4692.2014.06.011
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