Morphology and DNA barcoding of a newly intercepted fly species exoticin China, Calliphora dubia (Macquart, 1855)

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  • 1 School of Life Sciences, Sun Yat?Sen University, Guangzhou 510275, Guangdong Province, China;2 Zhongshan Entry-Exit Inspection and Quarantine Bureau, Zhongshan 528403, Guangdong Province, China

Received date: 2014-08-04

  Online published: 2014-12-20

Supported by

Supported by the National Key Technology R & D Program (No. 2012BAK11B05), Administration of Quality Supervision,Inspection and Quarantine Science and Technology Project (No. 2012IK223), Guangdong Entry?Exit Inspection and Quarantine Bureau Science and Technology Project (No. 2013GDK36, 2013GDK39) and the Zhongshan City Science and Technology Project (No. 20123A298)

Abstract

Objective To apply DNA barcoding in species identification of exotic vectors. Methods Morphology and DNA barcoding based identification was performed on two blowflies from Australia that were intercepted at the Shenwan port (Zhongshan, Guangdong) in September 2013. The identification results were confirmed with specialists in Australia and compared to data of a similar species, Calliphora augur. Results The two blowflies were identified as C. dubia, which represents exotic species in China based on sci?tech novelty search. Conclusion DNA barcoding is a strong supplementary tool for conventional taxonomy, which plays an important role in frontier health and quarantine inspection by timely identifying vector insects, reducing the spread of vector insects and the carried pathogens, and mitigating threats to public health.

Cite this article

LIAO Jun-lei, YUE Qiao-yun, QIU De-yi, WEI Xiao-ya, LIU De-xing, JIA Feng-long . Morphology and DNA barcoding of a newly intercepted fly species exoticin China, Calliphora dubia (Macquart, 1855)[J]. Chinese Journal of Vector Biology and Control, 2014 , 25(6) : 509 -513 . DOI: 10.11853/j.issn.1003.4692.2014.06.006

References

[1] 施宗伟,姚文国. 从口岸截获疫情浅析外来昆虫入侵特点和防范对策[J]. 昆虫知识,2004,41(4):371-374.
[2] Wallman JF. Third?instar larvae of common carrion?breeding blowflies of the genus Calliphora in South Australia[J]. Invertebr Taxion,2001,15(1):37-51.
[3] 谭玲,何建伟,王志杰,等. DNA条形码在国境卫生检疫中的应用[J]. 中国国境卫生检疫杂志,2009,32(5):425-431.
[4] 岳巧云,邱德义,胡佳,等. DNA条形码:医学媒介生物快速准确鉴定的利器[J]. 检验检疫学刊,2013,23(5):49,60-63.
[5] Hebert PDN, Cywinska A, Ball SL, et al. Biological identifications through DNA barcodes[J]. Philos Trans R Soc Lond B Biol Sci, 2003,270:313-321.
[6] Schindel DE, Miller SE. DNA Barcoding a useful tool for taxonomists[J]. Nature,2005,435:17.
[7] Gossner MM, Hausmann A. DNA barcoding enables the identification of caterpillars feeding on native and alien oak (Lepidoptera: Geometridae)[J]. Mitt Münch Ent Ges,2009,99:135-140.
[8] Vernooy R, Haribabu E, Muller MR, et al. Barcoding life to conserve biological diversity: beyond the taxonomic imperative[J]. PLoS Biol,2010,8(7):e1000417. doi:10.1371/journal.pbio.1000417.
[9] Taylor HR, Harris WE. An emergent science on the brink of irrelevance: a review of the past 8 years of DNA barcoding[J]. Mol Ecol Resour,2012,12(3):377-388.
[10] 岳巧云,邱德义,黄艺文,等. 应用DNA条形码技术鉴定未知双翅目蛹[J]. 中国国境卫生检疫杂志,2011,34(5):343-347.
[11] 王颖,王勇,高涛,等. DNA条形码技术用于原木携带蚊种的鉴定[J]. 中国国境卫生检疫杂志,2013,36(4):249-251.
[12] 贺骥,朱福春,苏永宽,等. DNA条形码技术应用于口岸吸血蠓类快速分子鉴定的研究[J]. 中华卫生杀虫药械,2014,20(2):131-135.
[13] Wallman JF. A key to the adults of species of blowflies in southern Australia known or suspected to breed in carrion[J]. Med Vet Entomol,2001,15(4):433-437.
[14] Folmer O, Black M, Hoeh W, et al. DNA primers for amplification of mitochondrial cytochrome c oxidase subunitⅠ from diverse metazoan invertebrates[J]. Mol Mar Biol Biotechnol,1994,3(5):294-299.
[15] Kumar S, Nei M, Dudley J, et al. MEGA: a biologist?centric software for evolutionary analysis of DNA and protein sequences[J]. Brief Bioinform,2008,9(4):299-306.
[16] Renaud AK, Savage J, Adamowicz SJ. DNA barcoding of Northern Nearctic Muscidae (Diptera) reveals high correspondence between morphological and molecular species limits[J]. BMC Ecol,2012, 12:24. doi:10.1186/1472-6785-12-24.
[17] Rivera J, Currie D. Identification of Nearctic black flies using DNA barcodes (Diptera:Simullidae)[J]. Mol Ecol Resour,2009,9 Suppl 1:S224-236.
[18] Schuehli GS, Carvalho CJB, Wiegmann BM. Molecular phylogenetics of the Muscidae (Diptera: Calyptratae): new ideas in a congruence context[J]. Invert Syst,2007,21:263-278.
[19] Wallman JF, Adams M. Molecular systematics of Australian carrion?breeding blowflies of the genus Calliphora (Diptera: Calliphoridae)[J]. Aust J Zool,1997,45:337-356.
[20] Mackerras IM, Fuller ME. A survey of the Australian sheep blowflies[J]. J Csir,1937,10:261-270.
[21] Lee DJ. Human myiasis in Australia[J]. Med J Aust,1968,1(5):170-173.
[22] Dayrat B. Towards integrative taxonomy[J]. Biol J Linn Soc Lond, 2005,85(3):407-415.
[23] Padial JM, Miralles A, De la Riva I, et al. The integrative future of taxonomy[J]. Front Zoo,2010,7:16.
[24] Bergsten J, Brilmyer G, Crampton?Platt A, et al. Sympatry and colour variation disguised well?differentiated sister species: Suphrodytes revised with integrative taxonomy including 5 kbp of housekeeping genes (Coleoptera: Dytiscidae)[J]. DNA Barcodes, 2012,1:1-18.
[25] Kurahashi H. The tribe Calliphorini from Australian and Oriental regions.Ⅱ. Calliphora?group (Diptera: Calliphoridae)[J]. Pacific Insects,1971,13:141-204.
[26] Hardy GH. Some Australian species of Calliphora (subgenera Neopollenia and Proekon)[J]. Bull Entomol Res,1932,23(4):549-558.
[27] Monzu N. Coexistence of Carrion?breeding Calliphoridae (Diptera) in Western Australia[D]. Perth:University of Western Australia,1977.
[28] Wallman JF, Donnellan SC. The utility of mitochondrial DNA sequences for the identification of forensically important blowflies (Diptera: Calliphoridae) in southeastern Australia[J]. Forensic Sci Int,2001,120(1/2):60-67.
[29] Wallman JF, Leys R, Hogendoorn K. Molecular systematics of Australian carrion?breeding blowflies (Diptera:Calliphoridae) based on mitochondrial DNA[J]. Invert Syst,2005,19(1):1-15.
[30] Harvey ML, Gaudieri S, Villet MH, et al. A global study of forensically significant calliphorids: implications for identification[J]. Forensic Sci Int,2008,177(1):66-76.
[31] 岳巧云,石先来. DNA条形码技术“查入侵”又快又准[N]. 中国国门时报,2010-12-09(3).
[32] Dasmahapatra KK, Elias M, Hill RI, et al. Mitochondrial DNA barcoding detects some species that are real, and some that are not[J]. Mol Ecol Resour,2010,10(2):264-273.
[33] DeSalle R, Egan MG, Siddall M. The unholy trinity: taxonomy, species delimitation and DNA barcoding[J]. Philos Trans R Soc Lond B Biol Sci,2005,360:1905-1916.
[34] Dupuis JR, Roe AD, Sperling FAH. Multi?locus species delimitation in closely related animals and fungi: one marker is not enough[J]. Mol Ecol,2012,21(18):4422-4436.
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