ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Efficacy of Bacillus thuringiensis serotype H-14 in controlling vector mosquitoes
Received date: 2014-06-04
Online published: 2014-12-20
Supported by
Supported by the National Natural Science Foundation of China (No. 81201317) and the Science and Technology Development Plan Project of Shandong Province (No. 2010GSF10207)
Objective To study the activity and efficacy of Bacillus thuringiensis serotype H-14 (B.t. H-14) against vector mosquitoes and the persistence of bacterial liquid preparation. Methods B.t. H-14 with high pathogenicity against mosquitoes was tested in the field sites. The densities of larval and adult mosquitoes were quantified by dippingand human-baited net trapping. The results were compared with the mosquito densities in an untreated control site to determine the field efficacy of B.t. H-14. The persistence of B. thuringiensis in different water bodies was observed in the laboratory. Results The relative population index (RPI) of vector mosquitoes was under 10 during peak periods, which indicated that B.t. H-14 lowered the populations of vector mosquitoes in natural habitats. The mosquito densities in the treated site remained at a relatively low level, with a larval density less than 50/dip and an adult density less than 20/hour. However, the density of mosquitoes in the untreated control site changed considerably with seasons, with a larval density more than 100/dip and an adult density up to 188/hour. In the laboratory, the activity of B.t. H-14 lasted 30 d. The larvicidal activity of B.t. H-14 was optimal in pure water; in polluted water, however, its activity reached the peakin one weekand then decreased gradually. Conclusion In the natural environment, B.t. H-14 can be used to control vector mosquitoes, and the concentration of bacterial liquid applied and the number of applications can be determined according to the mosquito population densities.
WEI Ji-bo, LI Gui-ling, MENG Qing-dong, LIU Li-juan, DAI Yu-hua, WANG Huai-wei . Efficacy of Bacillus thuringiensis serotype H-14 in controlling vector mosquitoes[J]. Chinese Journal of Vector Biology and Control, 2014 , 25(6) : 502 -505 . DOI: 10.11853/j.issn.1003.4692.2014.06.004
[1] 李士根,王兆杰,全芯,等. 山东省淡色库蚊对化学杀虫剂的抗性及防治对策[J]. 中国媒介生物学及控制杂志,2010,21(5):434-436.
[2] 孟凤霞,靳建超,陈云,等. 我国淡色库蚊/致倦库蚊对常用化学杀虫剂的抗药性[J]. 中国媒介生物学及控制杂志,2011,22(6):517-520,528.
[3] Kumar PM, Murugan K, Kovendan K, et al. Mosquito larvicidal and pupicidal efficacy of Solanum xanthocarpum (Family: Solanaceae) leaf extract and bacterial insecticide, Bacillus thuringiensis, against Culex quinquefasciatus Say (Diptera: Culicidae)[J]. Parasitol Res,2012,110(6):2541-2550.
[4] 徐健,赵松,刘琴. 苏云金杆菌以色列亚种生物杀蚊幼颗粒剂及其作用特性[J]. 中国血吸虫病防治杂志,2013,25(1):52-55.
[5] Darsi S, Prakash GD,Udayasuriyan V. Cloning and characterization of truncated cry1Ab gene from a new indigenous isolate of Bacillus thuringiensis[J]. Biotechnol Lett,2010,32(9):1311-1315.
[6] da Costa JR, Rossi JR, Marucci SC, et al. Toxic activity of Bacillus thuringiensis isolates to Aedes aegypti (L.) (Diptera: Culicidae) larvae[J]. Neotrop Entomol,2010,39(5):757-766.
[7] Balaraman K. Occurrence and diversity of mosquitocidal strains of Bacillus thuringiensis[J]. J Vector Borne Dis,2005,42(3):81-86.
[8] 陆宝麟. 蚊虫综合治理[M]. 北京:科学出版社,1999:118-123.
[9] Lee YW, Zairi J, Yap HH. Integration of Bacillus thuringiensis H-14 formulations and pyriproxyfen for the control of larvae of Aedes aegypti and Aedes albopictus[J]. J Am Mosq Control Assoc, 2005,21(1):84-89.
[10] 陈登宏,房天职,杨新史,等. 苏云金杆菌H-14对蚊幼虫的毒力测定及现场防制效果[J]. 医学动物防制,1991,7(3):222-225.
[11] 王美秀,王淑京,杨晓杰,等. 两种剂型苏云金杆菌血清14型制剂对埃及伊蚊幼虫的毒杀效果试验[J]. 中国媒介生物学及控制杂志,1996,7(1):70.
[12] 消毒杀虫灭鼠手册编写组. 消毒杀虫灭鼠手册[M]. 北京:人民卫生出版社,1980:218-219,319-320.
[13] Loke SR,Andy-Tan WA, Benjamin S, et al. Susceptibility of field-collected Aedes aegypti (L.) (Diptera: Culicidae) to Bacillus thuringiensis israelensis and temephos[J]. Trop Biomed,2010,27(3):493-503.
[14] Paris M, David JP, Despres L. Fitness costs of resistance to Bti toxins in the dengue vector Aedes aegypti[J]. Ecotoxicology,2011,20(6):1184-1194.
[15] 代玉华,黄晓丹,刘丽娟,等. 济宁地区淡色库蚊幼虫对苏云金杆菌的抗性水平研究[J]. 寄生虫与医学昆虫学报,2011,18(2):99-102.
[16] 鲁睿. 苏云金杆菌和麦蛾柔茧蜂对印度谷螟的联合控制作用研究[D]. 武汉:华中农业大学,2008.
[17] Ketseoglou I, Bouwer G. The susceptibility of five African Anopheles species to Anabaena PCC 7120 expressing Bacillus thuringiensis subsp. israelensis mosquitocidal cry genes[J]. Parasit Vectors,2012,5:220. doi:10.1186/1756-3305-5-220.
[18] 代玉华,程鹏,刘丽娟,等. 苏云金杆菌对不同龄期淡色库蚊幼虫的灭杀作用[J]. 中华卫生杀虫药械,2011,17(1):29-30.
[19] 易建荣,林立丰. 苏云金杆菌悬浮剂杀灭白纹伊蚊不同龄期幼虫实验及其影响因素分析[J]. 中华卫生杀虫药械,2003,9(2):15-18.
[20] 苏善强,甘保吉,陈惟吾,等. 苏云金杆菌H-14块状缓释剂控制家庭积水容器中埃及伊蚊幼虫的效果观察[J]. 中国媒介生物学及控制杂志,1995,6(6):404-407.
[21] 黄丽华,冯向阳,耿文奎. 氯氰菊酯与苏云金杆菌混合制剂和苏云金杆菌制剂对致乏库蚊幼虫的毒效比较[J]. 医学动物防制,2001,17(10):532-533.
[22] Armengol G, Hernandez J, Velez JG, et al. Long-lasting effects of a Bacillus thuringiensis serovar israelensis experimental tablet formulation for Aedes aegypti (Diptera: Culicidae) control[J]. J Econ Entomol,2006,99(5):1590-1595.
[23] Jacups SP, Rapley LP, Johnson PH,et al. Bacillus thuringiensis var. israelensis misting for control of Aedes in cryptic ground containers in north Queensland,Australia[J]. Am J Trop Med Hyg, 2013,88(3):490-496.
/
| 〈 |
|
〉 |