Objective To investigate the detection rate, serotype, and virulence gene distribution of Yersinia enterocolitica isolated from live pigs in Dengfeng, China, and to provide reference for the development of preventive measures. Methods Yersinia enterocolitica was isolated from the pharyngeal swabs (tonsils) and intestinal contents (at the ileocecal junction), which were collected from the live pigs in abattoirs in Dengfeng. Biochemical identification, serotyping, and PCR detection of virulence gene were performed on the isolates. Results A total of 107 strains of Y. enterocolitica were isolated from 196 pharyngeal swabs, with a detection rate of 54.59%; 36 strains were isolated from 196 intestinal contents, with a detection rate of 18.37%. The serotype of the isolates was O∶3. Three biological types were identified as follows: typeⅠ (ail＋, ystA＋, ystB－, yadA＋, virF＋), with a ratio of 93.71% (134/143), and typeⅡ (ail＋, ystA＋, ystB－, yadA－, virF－), with a ratio of 6.29% (9/143). Conclusion The pathogenic strains are dominant among Y. enterocolitica isolated from live pigs in Dengfeng. Monitoring of Y. enterocolitica should be strengthened in the future.
ZHANG Sheng-yong, XIA Sheng-li, WANG De-xiang, ZHANG Jin, LI Hong-min
. Etiological study of Yersinia enterocolitica isolated from live pigsin abattoirs in Dengfeng, China[J]. Chinese Journal of Vector Biology and Control, 2014
, 25(5)
: 459
-460
.
DOI: 10.11853/j.issn.1003.4692.2014.05.020
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