Objective To identify spotted fever group rickettsiae (SFGR) isolated from Dermacentor sinicus, which were collected from the hedgehogs in the Chinese Center for Disease Control and Prevention in Changping District of Beijing, China. Methods SFGR isolates were identified by morphological and molecular biological methods, followed by phylogenetic analysis. Results SFGR propagated in DH82 cells and caused significant cytopathic effects. After Gimenez staining, a large number of rickettsiae were clearly seen in dark red. Sequence analysis revealed that the 16S gene of the SFGR isolates was 100% identical to that of Rickettsia sibirica strain 246 and R. sibirica strain BJ-90. Phylogenetic analysis demonstrated that the SFGR isolates were located in the same evolutionary branch as R. sibirica, which further proved that they were R. sibirica. Conclusion The discovery of tick-born SFGR in Changping district provides etiological evidence for potential existence of the natural foci of North Asia fever in this area.
HE Jin-rong, LI Ming-hui, CHEN Xiao-ping, LI Ci-xiu
. Isolation and identification of Rickettsia sibirica carried by ticksin Changping district of Beijing, China[J]. Chinese Journal of Vector Biology and Control, 2014
, 25(5)
: 435
-437
.
DOI: 10.11853/j.issn.1003.4692.2014.05.013
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