Objective　To investigate the influence of containers on the insecticide resistance bioassay among Culex pipiens pallens larvae, and to determine whether disposable cups can replace glass beakers in these tests with the purpose of increasing experimental efficiency. Methods　Using wild Cx. pipiens pallens larvae, the susceptibility of larvae (LC50 with 95%CI) to deltamethrin, temephos, and BMPC was determined using the glass beaker, disposable plastic cup, or disposable paper cup, with impregnation method as recommended by WHO, and the LC50 values measured using different containers were compared. Results　The LC50 (95%CI) values of Cx. pipiens pallens larvae to deltamethrin measured using the glass beaker, disposable plastic cup, and disposable paper cup were 0.002 009 (0.001 654-0.002 449), 0.003 858 (0.003 062-0.005 224), and 0.005 459 (0.004 051-0.008 757) mg/L, respectively; there were significant differences in LC50 value between the glass beaker group and the other two groups. To temephos, the LC50 (95%CI) values were 0.000 955 (0.000 816-0.001 094), 0.003 007 (0.002 661-0.003 407), and 0.003 830 (0.003 450-0.004 256) mg/L, respectively; there were significant differences between the glass beaker group and the other two groups. To BMPC, the LC50 (95%CI) values were 0.3470 (0.3059-0.3821), 0.2575 (0.2118-0.3095), and 0.4844 (0.4460-0.5232) mg/L, respectively; there was a significant difference between the glass beaker group and disposable paper cup group, but no significant difference was found between the glass beaker group and disposable plastic cup group. The susceptibility of Cx. pipiens pallens larvae to deltamethrin was less concentration-dependent as compared with that to temephos; with increasing temephos concentration, susceptibility became less dependent on different types of containers. Conclusion　In the bioassay of Cx. pipiens pallens larvae’s resistance to insecticide, glass breaker is the best choice. Disposable cups may affect the results due to adsorption of pesticides. Special attention should be paid when using disposable cups instead of the glass beaker, and evaluation should be performed in these situations.