Objective To determine the susceptibility of Anopheles sinensis to DDT, deltamethrin, beta - cypermethrin,permethrin, and propoxur in Shenyang, China, and to provide a scientific basis for rational and effective use of pesticides in the control ofAn. sinensisand to reduce the outbreak of malaria in Shenyang area.Methods The adult mosquito filter paper contact method recommended by WHO was used in determination of resistance, given WHO distinguishing dosage. The insecticide resistance ofAn. sinensiswas evaluated by determination of resistance level, first knock-out time, and knock-out rates at different times. Results The mortality rates ofAn. sinensisafter 24 hours of treatment with deltamethrin, beta-cypermethrin, permethrin,propoxur, and DDT were 87% , 79% , 100% , 100% , and 100% , respectively.An. sinensiswas preliminarily resistant (M) to deltamethrin, resistant (R) to beta-cypermethrin, and susceptible (S) to DDT, permethrin, and propoxur. The median knock-out times (KT50) and the 95% confidence intervals were 24.4025 (27.2605-21.8442), 27.4794 (32.2923-23.3838), 19.6297(22.0655-17.4628), 9.0588 (9.5893-8.5578), and 23.8534 (26.1363-21.7699) min, respectively. The insecticide with the lowest KT50 was propoxur (9.0588 min), while the one with the highest KT50 was beta-cypermethrin (27.4794 min).Conclusion Anopheles sinensisin Shenyang has developed a certain level of resistance to beta-cypermethrin. Thus, it is necessary to use insecticides properly and to delay the development of insecticide resistance. An. sinensisis relatively susceptible to propoxur, indicating that propoxur can be applied as the major insecticide in the control ofAn. sinensisat the present time.