Objective To study the main hosts and vectors of tick-borne diseases in Jiande, Zhejiang province, China in terms of the population seasonality and pathogen infection and to provide a scientific basis for the prevention and control of tick-borne diseases.Methods Ticks were collected with white cloth or from the host animals. Small mammals were captured using cages (please specify traps used). The infection with tick-borne pathogens was detected by PCR.Results There wereHaemaphysalis longicornis, Ixodes sinensis, and Rhipicephalus haemaphysaloidesin Jiande, and H. longicorniswas the dominant species. The population density of ticks was the highest in June, as shown by tick collection with white cloth, while the tick-carrying rate was the highest in June, as shown by tick collection from the host animals. Anaplasma phagocytophilum, Babesia, Ehrlichia chaffeensis, Rickettsia, Bartonella, and Hantaan virus were detected in ticks and small mammals, and there were co-infections.Conclusion The hosts and vectors of tick-borne diseases are widely distributed in Jiande, and various pathogens are harbored by the tick species studies. Mitigation should be taken into consideration to prevent tick-borne diseases.