Objective To provide a scientific basis for confirmation of typical Lyme disease by serological analysis of the first suspected case of Lyme disease in Shanxi province, China and to improve the clinicians’recognition of Lyme disease for prompt diagnosis and treatment. Methods Using indirect immunofluorescence assay (IFA) and Western blot (WB), the patient suspected of Lyme disease was tested for anti-Borrelia burgdorferiIgM and IgG antibodies. A comprehensive analysis was conducted given clinical symptoms. Tests were repeated half a month later. Results In the first test, IgM antibodies were detected by IFA and WB. The titer of IgM antibodies was1∶64 in IFA, and two protein bands, P41 and P31, were present in WB.IgG antibodies were not found by any of the two tests. In the second test half a month later, IgG antibodies were positive in both IFA and WB. The titer of IgG antibodies went up to1∶256 in IFA, and two protein bands, P41 and P31, were present in WB. IgM antibodies were not found by any of the two approaches.Conclusion Based on the clinical history and progression of disease, as well as the serological results of this research, the diagnosis of Lyme disease can be confirmed, indicating the existence of typical Lyme disease in Shanxi province.
GENG Zhen, LI Guo-hua, HOU Xue-xia, ZHANG Lin, HAO Qin
. The first case of Lyme disease in Shanxi province, China: a case study[J]. Chinese Journal of Vector Biology and Control, 2014
, 25(4)
: 318
-319
.
DOI: 10.11853/j.issn.1003.4692.2014.04.008
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