Objective To identify the genus, species, and type of a bacterial strain (X175) isolated from a rodent of natural death.Methods The species and genus of X175 were identified based upon 16S rDNA sequencing. Real-time PCR was used in the detection of toxin genes commonly detected in diarrheagenic Escherichia coli(stx1/stx2/eae, lt/st, aggR/ipaH) and genes of E. coliO104 gene and H4 gene. Nine virulence genes ofenterohemorrhagicE. coli(EHEC) O104∶H4 were detected by PCR.Results The 16S rDNA sequence of X175 strain was 99% homogeneous to that of strains ofE.spp., and was most closely related toE. coliO104∶H4 in phylogenetic analysis.E. coliO104 gene andipaH were detected, whilestx1/stx2/eae, lt/st, aggR,Shigella/Salmonella, and E. coli H4 gene were not. None of the 9 virulence genes of EHEC O104∶H4 was detected.Conclusion X175 is an attenuated strain ofE. coliO104. H antigen and some other features remain to be further studied.
GUO Ying, CHEN Shuang-yan, SHI Li-yuan, DONG Shan-shan, WANG Peng
. Molecular identification of a bacterial strain isolated from a rodent of natural death[J]. Chinese Journal of Vector Biology and Control, 2014
, 25(4)
: 306
-308
.
DOI: 10.11853/j.issn.1003.4692.2014.04.005
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