Objective To perform a fundamental research on the classification and geographic distribution of Ceratopogonidae in Guangxi Zhuang Autonomous Region, China. Methods Based on the relevant literature on biting midges, 10 collecting sites were set in north, south, east, west, and central areas of Guangxi. Insects of Ceratopogonidae were collected by insect net, human?baited net trap, baited trap, and light trap. Insects were finally identified and classified. Results About ten thousand insects of Ceratopogonidae were collected from Guangxi in this study. Classification of insects covered 4 subfamilies, 12 genera, and 91 species, including 1 species of Leptoconops, 14 species of Dasyhelea, 10 species of Atrichopogon, 13 species of Forcipomyia, 12 species of Lasiohelea, 31 species of Culicoides, 7 species of Alluaudomyia, 1 species of Downeshelea, 3 species of Stilobezzia, 1 species of Guihelea, 1 species of Mackerrasomyia, and 5 species of Bezzia. Conclusion The classification and geographic distribution of Ceratopogonidae in Guangxi Zhuang Autonomous Region have basically been determined by this study. This study also provides a scientific basis for further studies of zoogeography and integrated control.