Application of 5S-23S rRNA and ospA genes in PCR?based detectionof Borrelia burgdorferi in rodent samples of Zhejiang province, China

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  • Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China;
    2 Pan’an Center for Disease Control and Prevention

Received date: 2013-12-30

  Online published: 2014-06-20

Abstract

Objective To evaluate the application of 5S-23S rRNA and outer ospA genes in PCR?based detection of Borrelia burgdorferi and to investigate the infection with B. burgdorferi in rodents of Zhejiang province, China. Methods PCR was used to amplify 5S-23S rRNA and ospA gene fragments from 100 mice collected from different areas of Pan’an county. PCR products were purified and sequenced. Results Of the 100 mice investigated, 3 were positive for 5S-23S rRNA gene of B. burgdorferi and 5 for ospA gene of B. burgdorferi. The sequences of PCR products were found with high homology to the reference genes. Conclusion B. burgdorferi can be detected from rodent samples by PCR amplification of 5S-23S rRNA and ospA genes.

Cite this article

JIANG Li-ping, WEI Yi-cheng, ZHANG Lei, YING Kai-man, ZHAN Li, LU Qun-ying . Application of 5S-23S rRNA and ospA genes in PCR?based detectionof Borrelia burgdorferi in rodent samples of Zhejiang province, China[J]. Chinese Journal of Vector Biology and Control, 2014 , 25(3) : 249 -253 . DOI: 10.11853/j.issn.1003.4692.2014.03.015

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