ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Effects of several metallic ions on activity of phenoloxidasefrom Oncomelania hupensis of different ages
Received date: 2013-12-26
Online published: 2014-06-20
Objective To investigate the effects of several metallic ions on the activity of phenoloxidase (PO) from Oncomelania hupensis of different ages such as adult snails, young snails, and snail eggs. Methods PO from O. hupensis of different ages was partially purified. PO activity was determined with different concentrations of NaCl, KCl, Na2SO4, K2SO4, MgSO4, CaSO4, ZnSO4, and CuSO4. Catechol was used as a substrate. Results Na+ and K+ had no effects on the activity of PO from O. hupensis of any age. Mg2+ and Ca2+ showed enhancing effects in all age groups, while Zn2+ had an inhibitory effect. For O. hupensis of different ages, Cu2+ enhanced the enzyme activity at low concentrations, but inhibited the enzyme activity at high concentrations. Conclusion Several metallic ions such as Mg2+, Ca2+, Zn2+, and Cu2+ can affect the activity of PO from O. hupensis of different ages. These findings provide a rationale for developing novel methods in chemically eliminating snails.
Key words： Oncomelania hupensis; Phenoloxidase; Metallic ion; Enzyme activity
YANG Jin-sun, ZHOU Shu-lin . Effects of several metallic ions on activity of phenoloxidasefrom Oncomelania hupensis of different ages[J]. Chinese Journal of Vector Biology and Control, 2014 , 25(3) : 246 -248 . DOI: 10.11853/j.issn.1003.4692.2014.03.014
[1] 仝彩玲, 李培英, 周金林. 莱姆病诊断技术研究进展[J]. 中国动物传染病学报, 2009, 17(3):76.
[2] 陈永金, 张智德, 叶楣进. 莱姆病例报告[J]. 浙江预防医学与疾病监测, 1992, 4(3):2.
[3] Masuzawa T, Fukui T, Miyake M, et al. Determination of members of a Borrelia afzelii?related group isolated from Ixodes nipponensis in Korea as Borrelia valaisiana[J]. Int J Syst Bacteriol, 1999(4):1409-1415.
[4] Chu CY, Liu W, Jiang BG, et al. Novel genospecies of Borrelia burgdorferi sensu lato from rodents and ticks in Southwestern China[J]. J Clin Microbiol, 2008, 46(9):3130-3133.
[5] 姜理平, 郑寿贵, 莫世华, 等. 浙江省在鼠形动物中检测到伯氏疏螺旋体[J]. 中国媒介生物学及控制杂志, 2008, 19(5):461-463.
[6] Chu CY, Jiang BG, Qiu EC, et al. Borrelia burgdorferi sensu lato in sheep keds (Melophagus ovinus), Tibet, China[J]. J Vet Microbiol, 2011, 49(3):526-529.
[7] 褚宸一, 何静, 赵秋敏, 等. 我国部分林区鼠中莱姆病螺旋体的分子流行病学调查研究[J].中国人兽共患病学报, 2006, 22(9):817-818.
[8] 窦晓光. 传染病学[M]. 北京:人民卫生出版社, 2008:255-259.
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