ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
A survey of species composition and population dynamics of mosquitoesin Lhasa, Tibet, China from 2009 to 2013
Received date: 2014-02-24
Online published: 2014-06-20
Objective To investigate the species composition and population dynamics of mosquitoes in Lhasa, Tibet, China, so as to provide scientific evidence for the surveillance, prevention, and control of mosquitoes and mosquito?borne diseases in this city. Methods Representative research sites were selected based on geographical distributions in Chengguan District in Lhasa. Adult mosquitoes were collected by bed net traps, labor hour method, and light traps in 2009, 2012, and 2013. The trapped mosquitoes were initially identified according to morphological criteria. A proportion of them were examined using a multiplex PCR assay for species confirmation. Results Mosquitoes in Lhasa comprised the subspecies of Culex pipiens complex and Armigeres subalbatus, with subspecies of Cx. pipiens complex as the dominant species. The mean mosquito density monitored by bed net traps was 19.57 mosquitoes per net?hour in 2009 and 3.06 mosquitoes per net?hour in 2012; there was no significant difference in mosquito density between research sites in different years (F1, 1＝0.934, P＞0.05). The mean mosquito density monitored by labor hour method was 62.10 mosquitoes per person?hour in 2009 and 7.90 mosquitoes per person?hour in 2012; there was no significant difference in mosquito density between research sites in different years (F1, 1＝0.874, P＞0.05). The mean mosquito density monitored by light traps was 1.47 mosquitoes per trap?hour in 2012 and 0.20 mosquitoes per trap?hour in 2013; there was no significant difference in mosquito density between research sites in different years (F1, 3＝1.503, P＞0.05). A hundred and five mosquitoes captured in 2012 were identified by multiplex PCR, of which 36 (34.29%) were homozygous subspecies of Cx. pipiens complex, while 69 (65.71%) were hybrids. Conclusion Mosquitoes of Cx. pipiens complex subspecies are established in Lhasa, and the population density was relatively low in recent years, indicating that the risk of mosquitoes and mosquito?borne diseases exists in Lhasa. Therefore, surveillance and risk assessment should be conducted to provide a basis for decision?making in the control of mosquitoes and mosquito?borne diseases in this region.
LIU Xiao-bo, Cirendunzhu, GUO Yu-hong, Pengcuociren, BAI Li, SANG Shao-wei, Baimaciwang, GU Shao-hua, Dazhen, CHEN Bin, ZHOU Lin, WAN Fang-jun, HU Ya-ping, XU Jun-fang, WANG Jun, Dawa, LI Gui-chang, Xiraoruodeng, LIU Qi-yong . A survey of species composition and population dynamics of mosquitoesin Lhasa, Tibet, China from 2009 to 2013[J]. Chinese Journal of Vector Biology and Control, 2014 , 25(3) : 200 -204 . DOI: 10.11853/j.issn.1003.4692.2014.03.002
[1] Liu Q, Liu X, Cirendunzhu, et al. Mosquitoes established in Lhasa city, Tibet, China [J]. Parasite Vector, 2013, 6(1):224.
[2] 陆宝麟, 吴厚永. 中国重要医学昆虫分类与鉴别[M]. 郑州:河南科学技术出版社, 2003:16-46, 388-426.
[3] Sanogo YO, Kim CH, Lampman R, et al. Identification of male specimens of the Culex pipiens complex (Diptera:Culicidae) in the hybrid zone using morphology and molecular techniques[J]. J Med Entomol, 2008, 45(2):203-209.
[4] Smith JL, Fonseca DM. Rapid assays for identification of members of the Culex (Culex) pipiens complex, their hybrids, and other sibling species (Diptera:culicidae)[J]. Am J Trop Med Hyg, 2004, 70(4):339-345.
[5] 赵彤言, 陆宝麟. 中国尖音库蚊复合组杂交的研究[J]. 动物分类学报, 1996, 21(2):196-223.
[6] 郭玉红, 刘起勇, 尹遵栋, 等. 西藏林芝地区蚊媒初步调查研究[J]. 中国媒介生物学及控制杂志, 2010, 21(4):300-302.
[7] 李佳, 周红宁, 王丕玉, 等. 澜沧江中下游地区居民点蚊虫多样性的初步研究[J]. 中国媒介生物学及控制杂志, 2009, 20(2):104-107.
[8] 景晓, 霍新北, 常树珍, 等. 蒙山不同海拔高度蚊虫群落结构特点的比较研究[J]. 中国媒介生物学及控制杂志, 2009, 20(1):18-20.
[9] Fonseca DM, Keyghobadi N, Malcolm CA, et al. Emerging vectors in the Culex pipiens complex[J]. Science, 2004, 303(5663):1535-1538.
[10] Farajollahi A, Fonseca DM, Kramer LD, et al. “Bird biting” mosquitoes and human disease: a review of the role of Culex pipiens complex mosquitoes in epidemiology[J]. Infect Genet Evol, 2011, 11(7):1577-1585.
[11] Diaz?Badillo A, Bolling BG, Perez?Ramirez G, et al. The distribution of potential West Nile virus vectors, Culex pipiens pipiens and Culex pipiens quinquefasciatus (Diptera:Culicidae), in Mexico city[J]. Parasite Vector, 2011, 4:70.
[12] 于萍, 魏荣, 王志亮, 等. 西尼罗病毒蚊媒的种类、研究进展及监控措施[J]. 中国媒介生物学及控制杂志, 2005, 16(4):324-327.
[13] Barr AR. Occurrence and distribution of the Culex pipiens complex [J]. Bull World Health Organ, 1967, 37(2):293-296.
[14] Urbanelli S, Silvestrini F, Reisen WK, et al. Californian hybrid zone between Culex pipiens pipiens and Cx. pipiens quinquefasciatus revisited (Diptera:Culicidae) [J]. J Med Entomol, 1997, 34(2):116-127.
[15] Kothera L, Zimmerman EM, Richards CM, et al. Microsatellite characterization of subspecies and their hybrids in Culex pipiens complex (Diptera:Culicidae) mosquitoes along a north?south transect in the central United States[J]. J Med Entomol, 2009, 46(2):236-248.
[16] Humeres SG, Almiron WR, Sabattini MS, et al. Estimation of genetic divergence and gene flow between Culex pipiens and Culex quinquefasciatus (Diptera:Culicidae) in Argentina[J]. Mem I Oswaldo Cruz, 1998, 93(1):57-62.
[17] Fonseca DM, Smith JL, Kim HC, et al. Population genetics of the mosquito Culex pipiens pallens reveals sex?linked asymmetric introgression by Culex quinquefasciatus[J]. Infect Genet Evol, 2009, 9(6):1197-1203.
[18] Cui F, Qiao CL, Shen BC, et al. Genetic differentiation of Culex pipiens (Diptera: Culicidae) in China[J]. Bull Entomol Res, 2007, 97(3):291-297.
[19] 宋社吾, 赵彤言, 蒋书楠, 等. 我国尖音库蚊复合组蚊虫核糖体DNA第2内转录间隔区序列测定与分析[J]. 寄生虫与医学昆虫学报, 2003, 10(2):74-82.
[20] Zhao TY, Lu BL. A new record of Culex pipiens molestus in China and studies of autogeny and systematics[J]. China J Vector Bio Control, 1993, 4(4):241-243.
[21] Li YX, Li MH, Fu SH, et al. Japanese encephalitis, Tibet, China [J]. Emerg Infect Dis, 2011, 17(5):934-936.
[22] 何昌华, 赵伟, 王善青, 等. 海南省城区2012年蚊虫密度及季节消长分析[J]. 中国媒介生物学及控制杂志, 2014, 25(1):15-17.
[23] Benitez MA. Climate change could affect mosquito?borne diseases in Asia[J]. Lancet, 2009, 373(9669):1070.
[24] Li B, Cirendunzhu, Pengcuociren, et al. Rapid warming in Tibet, China:public perception, response and coping resources in urban Lhasa[J]. Environ Health Globe, 2013, 12(1):71.
[25] McAbee RD, Green EN, Holeman J, et al. Identification of Culex pipiens complex mosquitoes in a hybrid zone of West Nile virus transmission in Fresno county, California[J]. Am J Trop Med Hyg, 2008, 78(2):303-310.
[26] Patz JA, Campbell?Lendrum D, Holloway T, et al. Impact of regional climate change on human health[J]. Nature, 2005, 438(7066):310-317.
[27] Ruiz MO, Chaves LF, Hamer GL, et al. Local impact of temperature and precipitation on West Nile virus infection in Culex species mosquitoes in northeast Illinois, USA[J]. Parasite Vector, 2010, 3(1):19.
[28] Easton ER. Urbanization and its effects on the ecology of mosquitoes in Macau, Southeast Asia[J]. J Am Mosq Control Assoc, 1994, 10(4):540-544.
[29] Juliano SA, Lounibos LP. Ecology of invasive mosquitoes: effects on resident species and on human health[J]. Ecollett, 2005, 8(5):558-574.
/
| 〈 |
|
〉 |