Objective To understand Wolbachia distribution and genotype among mosquito populations in Guangdong. Methods PCRs were performed on mosquitoes from Culex, Aedes, Anopheles and Armigeres based on Wolbachia wsp gene, PCR products were sequenced and performed phylogenetic analysis using molecular biology software (Mega 5.2). Results Twelve samples were positive on Cx. pipiens quinquefasciatus, Ar. subalbatus, Ae. albopictus and unidentified Culex, 11 of them were successfully sequenced, phylogenetic analysis revealed they were divided into 5 clades belonged to Wolbachia supergroup A and supergroup B. Conclusion Both supergroup A and supergroup B were detected among Cx. pipiens quinquefasciatus, Ar. subalbatus, Ae. albopictus, but not in Ae. aegypti and Anopheles sinensis samples.
LIN Lifeng, WU De, ZHANG Huan, KOU Jing, ZHOU Huiqiong, DUAN Jinhua, WU Jun, LIU Wenhua
. Preliminary studies on infection of Wolbachia in mosquito populationsin Guangdong province, China[J]. Chinese Journal of Vector Biology and Control, 2014
, 25(2)
: 113
-115
.
DOI: 10.11853/j.issn.1003.4692.2014.02.006
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