Objective To observe the molluscicidal effect of a novel molluscicide, 10% wettable powder of quinoid-2′,5-dichloro-4′-nitrosalicylanilide (LDS), on Oncomelania hupensis from the mountainous areas in Zhejiang province, China in the laboratory and field and to provide a scientific basis for the application of LDS in the field. Methods O. hupensis was immersed in 0.1, 0.2, 0.4, 0.6, or 0.8 mg/L LDS in the laboratory and field; 0.2, 0.4, 0.6, 0.8, or 1.0 g/m2 LDS was sprayed onto O. hupensis in the laboratory and field, or powder form of LDS was scattered onto O. hupensis in the field. Meanwhile, 50% wettable powder of niclosamide ethanolamine salt (WPN) and clean water were used as controls. The molluscicidal effects were evaluated. Results O. hupensis had mortality rates of 100% and over 95% after being immersed in 0.1 mg/L LDS for one day in the laboratory and field, respectively, with no significant differences compared without O. hupensis treated with WPN (P＞0.05). O. hupensis had a mortality of over 95% after being sprayed with 0.8 g/m2 LDS for one day in the laboratory, a mortality of over 85% after being sprayed with 0.2 g/m2 LDS for three days in the field, and a mortality of over 85% after being scattered with 0.4 g/m2 LDS powder for one day in the field. The mortality of O. hupensis increased as the concentration of LDS rose and the exposure to LDS was prolonged. Conclusion LDS (10%) has a good molluscicidal effect on O. hupensis from mountainous areas of Zhejiang province in the laboratory and field.