RT-PCR detection and N gene sequence analysis of Lymphocytic choriomeningitis virus in rodents

HU Qun, QIU Jiong-liang, MA Si-jie, MA Yan, LI Jun

doi:10.11853/j.issn.1003.4692.2013.06.002

Chinese Journal of Vector Biology and Control >

2013 , Vol. 24 >Issue 6: 483 - 485

DOI: https://doi.org/10.11853/j.issn.1003.4692.2013.06.002

Original reports

RT-PCR detection and N gene sequence analysis of Lymphocytic choriomeningitis virus in rodents

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1 Daxie Exit-Entry Inspection and Quarantine Bureau, Ningbo 315812, Zhejiang Province, China;
2 Ningbo Exit-Entry Inspection and Quarantine Bureau

Received date: 2013-07-09

Online published: 2013-12-20

Supported by

Supported by the Science Project of Administration of Quality Supervision, Inspection and Quarantine(No. 2012IK233)and the Natural Science Foundation of Ningbo(No. 2013A610240)

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Abstract

Objective To determine the prevalence and gene sequence of Lymphocytic choriomeningitis virus (LCMV) in the rodents in Ningbo Meishan Port Area. Methods Two pairs of specific primers were designed according to LCMV N gene, and RT-PCR was used to detect LCMV in the rodent samples captured in Ningbo Meishan Port Area in 2012. The PCR products of positive samples were subject to sequencing and then to nucleotide homology and phylogenetic tree analyses. Results Fifty-three rodent samples were tested, and two samples from house mice Mus musculus were LCMV-positive. The two isolated LCMV strains had a homology of 75%-87% with the LCMV strains isolated in other countries; they had the highest homology (87%) with the strain WHI and belong to the same group in the phylogenetic tree as the strain WHI. Conclusion LCMV prevalence in local rodents is confirmed, and there are some genetic differences between the two newly detected LCMV strains and the LCMV strains isolated elsewhere.

Key words： Rodent; Lymphocytic choriomeningitis virus; RT-PCR; Sequence analysis

Cite this article

HU Qun, QIU Jiong-liang, MA Si-jie, MA Yan, LI Jun . RT-PCR detection and N gene sequence analysis of Lymphocytic choriomeningitis virus in rodents[J]. Chinese Journal of Vector Biology and Control, 2013 , 24(6) : 483 -485 . DOI: 10.11853/j.issn.1003.4692.2013.06.002

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