Objective To investigate the invasions of bedbugs and other disease vectors on an entry ship and to evaluate the results of disinsection. Methods The bedbugs and other disease vectors on an entry ship from abroad were investigated by visual observation and the collected specimens were identified. The introduced bedbugs and other disease vectors on the ship were killed by either residual spray, space spray or slit spraying with 0.5% Malathion water solution. Poisonous baits for cockroaches were put in the cabins at the same time. The results of disinsection were assessed. Results More than 300 specimens of bedbugs were collected in crews cabins and considered Cimex hemipterus (Fabricius, 1803). The mean densities of bedbugs were 180 bedbugs per man-hour collection and 300 bedbugs per square meter. Seventeen other species of disease vectors, including cockroaches, mosquitoes and flies were also collected on board. The result of disinsection was found 100% after treatment. Conclusion It is recommended to enhance quarantine inspection on entry ships. For the entry ships invaded by bedbugs and other disease vectors, comprehensive insecticide application should be adopted according to the type of vectors and their habitats.