Objective To analyze the epidemiological characteristics of new Bunia virus infections among hosts and vectors in Zhoushan Island area and to provide scientific evidence for their prevention and control. Methods Case surveillance was performed in accordance with the Ministry of Health Prevention Guide. In places with positive cases, ticks were collected using cloth flag method or from the body of rodents. Murine-like animals were captured every 3-months, using rodent cages; they were identified, and then their liver, spleen, blood, and lung were collected under aseptic conditions; RNA was extracted and subjected to fluorescence quantitative PCR. Twenty-five serum samples of cattle, sheep, and other animals were collected. A database was created using EpiData 3.1, and statistical analysis was performed using Excel 2003. Results In Daishan county, Zhejiang province, China, from 2011 to 2012, 24 human cases of new Bunia virus infection (in 11 males and 13 females) were confirmed, including 2 deaths; the minimum age was 47 years, and the maximum age was 85 years; 70.83% of all cases were aged over 60 years. Four cases had a history of tick bite. These cases were sporadic, with one case of familial clustering of infection and one case of imported infection. A total of 111 rodents were captured in the field, and 251 ticks were collected by either cloth flag method or from the body of animals. New Bunia virus nucleic acid was detected in ticks. Two new Bunia virus strains were isolated from the blood of two human cases; the S genes of virus strains had a high degree of homology, but with some difference from those of new Bunia viruses from six other provinces in China. Conclusion In recent years, there have been many cases of severe fever with thrombocytopenia syndrome in Daishan county, and new Bunia virus was detected in ticks. Thus, measures should be taken to enhance the study and surveillance of this disease.