ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
High-throughput xMAP suspension arrays for simultaneous detection of 9 tick-borne pathogens
Received date: 2013-04-21
Online published: 2013-10-20
Supported by
Supported by the International Cooperation Project of Ministry of Science and Technology (No. 2010DFA34250) and the Basic Scientific Research Project of Chinese Academy of Inspection and Quarantine (No. 2012JK016)
Objective To develop xMAP suspension arrays for simultaneous detection of tick-borne pathogens including forest encephalitis virus, Xinjiang hemorrhagic fever virus, spotted fever group rickettsiae, Babesia spp., Ehrlichieae, Francisella tularensis, Coxiella burneti, Borrelia burgdorferi, and Bartonella. Methods The 9 tick-borne pathogens were divided into two groups, and a multiplex PCR system was designed with up-primers labelled with anti-TAG and down-primers labelled with biotin. The PCR products were hybridized to the magnetic microspheres coupled with x-TAG. The conjugates were tested using the Bio-plex 100 system, and the 9 tick-borne pathogens were subjected to single and multiple detections and analyses. Results The mean fluorescence intensity three times higher than that of the background was judged as positive reaction. The results of single-plex and multiplex assays for the pathogen cultures demonstrated specific positive signals, without cross reaction, and the stability, sensitivity, and specificity of the method was good. Conclusion The liquid biochip technology platform with the beads coupled with x-TAG, which can simultaneously detect 9 tick-borne pathogens, has been successfully established. This platform holds promise for detection of tick-borne pathogens.
Key words： Suspension array; Tick-borne pathogen; Simultaneous detection; x-TAG
WANG Wang, YANG Yu, WANG Jing, ZHAO Ting-ting, SUN Xiao-hong, LIU Li-juan . High-throughput xMAP suspension arrays for simultaneous detection of 9 tick-borne pathogens[J]. Chinese Journal of Vector Biology and Control, 2013 , 24(5) : 397 -401 . DOI: 10.11853/j.issn.1003.4692.2013.05.005
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