ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Determination and analysis of complete genomic sequences of three Dengue 2 virus strains isolated in Guangdong province, China
Received date: 2013-04-26
Online published: 2013-10-20
Supported by
Supports by the National Institutes of Health Project (No. 1R01AI083202-01A1), National Science and Technology Major Project of China (No. 2012ZX10004-213-005), Guangdong Science and Technology Department Program (No. 2011B031500011), Guangzhou Science and Technology Department Program (No. 2010Y1-C151, 2012Y2-00020) and Science and Technology Program of Guangzhou Health Department (No. 201102A212006, 20121A011122)
Objective To determine the complete genomic sequences of 3 Dengue 2 virus (DEN-2) strains isolated in Guangdong province, China and to investigate their genotypes and sources. Methods The complete genomic sequences of 3 DEN-2 strains (GD09/93, GD05/98, and GD19/2001) from Guangdong province were established by RT-PCR amplification. The phylogenetic tree of DEN-2 was constructed by Kimura method. Results The complete genomic sequences of 3 DEN-2 strains were 10 723 nucleotides (nt) in length and contained a single long open reading frame (ORF) of 10 173 nucleotides (97-10 269 nt), encoding 3391 amino acids. The ORF encoded structural and non-structural proteins, flanked by 5' and 3' non-coding regions. Comparing GD05/98 with GD09/93, GD05/98 with GD19/2001, and GD09/93 with GD19/2001, the nucleotide sequence homologies were 93.3%, 92.4%, and 97.6%, respectively, and the deduced amino acid sequence homologies were 96.7%, 96.5%, and 98.5%, respectively. Conclusion The DEN-2 strains are pathogenic in suckling mice. Compared with DEN-2-04 strain (nonpathogenic in suckling mice), there are 18 amino acid substitutions that confer changes in charge or polarity. The charge changes at PrM-134, NS2A-153, and NS4B-102 have a relatively strong impact on the antigenicity. GD05/98 strain falls within the groupⅡ comprising Thailand strains, while GD09/93 and GD19/2001 strains fall within the group Ⅳ comprising strains from Indonesia, Australian and Taiwan. There are different DEN genotypes in China, and one genotype of DEN-2 may be transmitted in different periods.
BAI Zhi-jun, ZHANG Pei, HONG Wen-yan, LIU Jian-wei, DI Biao, YANG Zhi-cong, REN Rui-wen, FANG Mei-yu, LIN Li-hui . Determination and analysis of complete genomic sequences of three Dengue 2 virus strains isolated in Guangdong province, China[J]. Chinese Journal of Vector Biology and Control, 2013 , 24(5) : 392 -396 . DOI: 10.11853/j.issn.1003.4692.2013.05.004
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