ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Molecular epidemiological study of tick-borne spotted fever group Rickettsia in western mountain area of Hebei province, China
Received date: 2013-04-24
Online published: 2013-08-20
Objective To investigate the prevalence of tick-borne spotted fever group Rickettsia (SFGR) in the western mountain area of Hebei province, China and to provide a scientific basis for the prevention and control of tick-borne SFGR. Methods One set of specific primers, designed according to the outer membrane protein A (OmpA) gene sequence of SFGR, were used to amplify the OmpA gene in DNA samples extracted from Haemaphysalis longicornis collected in the western mountain area of Hebei province, and the positive samples were subjected to sequencing and sequence analysis to establish the molecular phylogenetic tree. Results Of 1227 DNA samples, 91 (7.42%) were positive for SFGR. The phylogenetic analysis based on nucleotide sequence showed that all detected SFGR strains were clustered together with Candidatus Rickettsia hebeiii (accession numbers: HQ651815, HQ651817, HQ651818, HQ651819, HQ651823, and HQ651824) and Rickettsia sp. Fujian strain FUJ (accession number: AF169629); these strains had the highest homology with Candidatus R. hebeiii (99.02% ), followed by Rickettsia sp. Fujian strain FUJ (98.50%), Rickettsia sp. Suifen strain HLJ-054 (accession number: AF179362) and Rickettsia sp. Hulin strain HL-93 (accession number: AF179364) (98.13% ), and Rickettsia sp. Japanese strain YM (accession number: U43795) (97.92%). Conclusion Tick-borne SFGR is prevalent in the western mountain area of Hebei province. Preventive measures should be taken in time to protect humans and animals from this disease.
FENG Shuai, WU Han, ZHANG Li-wen, LU Chao-xu, ZHANG Cheng-long, LI Zhi-ping, ZHANG Rong-zhen, ZHOU Hui . Molecular epidemiological study of tick-borne spotted fever group Rickettsia in western mountain area of Hebei province, China[J]. Chinese Journal of Vector Biology and Control, 2013 , 24(4) : 308 -312 . DOI: 10.11853/j.issn.1003.4692.2013.04.008
[1] 李朝品. 医学蜱螨学
[M]. 北京:人民军医出版社,2005:83-99.
[2] Grasperge BJ,Wolfson W,Macaluso KR. Rickettsia parkeri infection in domestic dogs, Southern Louisiana,USA,2011
[J]. Clin Microbiol Rev,2005,18:719-756.
[3] Boretti FS, Perreten A, Meli ML, et al. Molecular investigations of Rickettsia helvetica infection in dogs, foxes, humans, and Ixodes ticks
[J]. Appl Environ Microbiol,2009,75(10):3230-3237.
[4] 黄海楠,丁壮,何静,等. 部分省区啮齿动物中斑点热群立克次体的检测
[J]. 中国媒介生物学及控制杂志,2006,17(2):83-85.
[5] 邹亚学,贾青辉,刘朋朋,等. 唐山地区蜱传斑点热群立克次体分离流行病学调查
[J]. 中国兽医学报,2011,31(12):1729-1732.
[6] Zou Y, Wang Q, Liu P, et al. Detection of spotted fever group Rickettsia in Haemaphysalis longicornis from Hebei province, China
[J]. J Parasitol,2011,97(5):960-962.
[7] Zhang JZ, Fan MY, Wu YM, et al. Genetic classification of Rickettsia heilongjiangii and Rickettsia hulinii, two Chinese spotted fever group Rickettsiae
[J]. J Clin Microbiol,2000,38(9): 3498-3501.
[8] Roux V, Fournier PE, Raoult D. Differentiation of spotted fever group Rickettsiae by sequencing and analysis of restriction fragment length polymorphism of PCR amplified DNA of the gene encoding the protein romp A
[J]. J Clin Microbiol,1996,34(9):2058-2065.
[9] 喻艳林,杨进孙,芮景. 人粒细胞无形体病的诊治进展
[J]. 中华临床药理学与治疗学,2007,12(9):980-983.
[10] Zhang L, Liu Y, Ni D, et al. Nosocomial transmission of human granulocytic anaplasmosis in China
[J]. JAMA,2008,300(19):2263-2270.
[11] Yu XJ, Liang MF, Zhang SY, et al. Fever with thrombocytopenia associated with a novel Bunyavirus in China
[J]. N Engl J Med, 2011,364(16):1523-1532.
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