Isolation and identification of Hantavirus carried by rodents in Zhejiang province, China from 2008 to 2011

XU Fang, ZHU Han-ping, YAO Ping-ping, HU Dan, ZHANG Yun, XIE Rong-hui, YANG Zhang-nyu, QIAN Lei, ZHU Jin

doi:10.11853/j.issn.1003.4692.2013.04.001

Chinese Journal of Vector Biology and Control >

2013 , Vol. 24 >Issue 4: 285 - 288

DOI: https://doi.org/10.11853/j.issn.1003.4692.2013.04.001

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Isolation and identification of Hantavirus carried by rodents in Zhejiang province, China from 2008 to 2011

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1 Zhejiang Center for Disease Control and Prevention, Hangzhou 310051, Zhejiang Province, China;
2 The Institute of Military Medicine, Nanjing PLA Command, Nanjing 210002, Jiangsu Province, China

Received date: 2013-03-29

Online published: 2013-08-20

Supported by

Foundation(No. Y205749)and Jiangshu Provincial Natural Science Foundation(No. BK2010537)

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Abstract

Objective To analyze the prevalence and genotypes of Hantavirus (HV) among rodents in the epidemic areas of hemorrhagic fever with renal syndrome (HFRS) in Zhejiang province, China from 2008 to 2011. Methods Direct immunofluorescence assay and RT-PCR were used to detect the antigen and nucleic acid of HV. Mongolian gerbils and Vero-E6 cells were used to isolate viruses from the HV-positive rodents, and the M segments of HV isolates were subjected to sequence analysis. Results Apodemus agrarius was the dominant species of the rodents captured in the epidemic areas of HFRS in Zhejiang province from 2008 to 2011. Six strains of HV were isolated from the lungs of this rodent species. The sequence analysis showed that the six isolates belonged to Hantaan virus (HTNV), wherein Z521, 524, and 534 were classified as H7 subtype, and TT-27, T-43, and R88 might be classified as a new subtype. The six HV isolates showed high gene homology between them and with the strains previously isolated in Zhejiang province. Conclusion HTNV was prevalent in rodent in the epidemic areas of HFRS in Zhejiang province from 2008 to 2011.

Key words： Hantavirus; Isolation; M segment; Sequence analysis; Rodent

Cite this article

XU Fang, ZHU Han-ping, YAO Ping-ping, HU Dan, ZHANG Yun, XIE Rong-hui, YANG Zhang-nyu, QIAN Lei, ZHU Jin . Isolation and identification of Hantavirus carried by rodents in Zhejiang province, China from 2008 to 2011[J]. Chinese Journal of Vector Biology and Control, 2013 , 24(4) : 285 -288 . DOI: 10.11853/j.issn.1003.4692.2013.04.001

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