Chinese Journal of Vector Biology and Control ›› 2022, Vol. 33 ›› Issue (5): 666-671.DOI: 10.11853/j.issn.1003.8280.2022.05.010

• Experimental Study • Previous Articles     Next Articles

Investigation on resistance and resistance-conferring mutations in Anopheles sinensis in Sanya,Hainan province,China

WU Qun1, SUN Ding-wei1, LI Shan-gan1, ZENG Xue-xia1, ZENG Lin-hai1, WANG Shao-ping2, WU De-lei2, WEI Jia-jia2, LI Ling-gang3, CHEN Cheng-rong3, LIU Ying1, CHEN Yan1   

  1. 1. Department of Tropical and Chronic Disease Prevention and Control, Hainan Center for Disease Prevention and Control, Haikou, Hainan 570203, China;
    2. Sanya Center for Disease Prevention and Control, Sanya, Hainan 572000, China;
    3. Gaofeng Health Center of Tianya District of Sanya City, Sanya, Hainan 572022, China
  • Received:2022-03-29 Online:2022-10-20 Published:2022-10-14
  • Supported by:
    Hainan Province Science and Technology (No.ZDKJ2021035);Natural Science Foundation of Hainan Province of China (No.821MS0844,821QN420)

海南省三亚市中华按蚊抗药性及抗性相关基因突变调查

吴群1, 孙定炜1, 李善干1, 曾雪霞1, 曾林海1, 王绍萍2, 吴德雷2, 魏家佳2, 黎灵刚3, 陈成荣3, 刘莹1, 陈言1   

  1. 1. 海南省疾病预防控制中心热带病与慢性病预防控制所, 海南海口 570203;
    2. 三亚市疾病预防控制中心, 海南 三亚 572000;
    3. 三亚市天涯区高峰卫生院, 海南三亚 572022
  • 通讯作者: 陈言,E-mail:jkban702@163.com
  • 作者简介:吴群,女,硕士,助理研究员,主要从事病媒生物防制工作,E-mail:wuqunreceptor@163.com;孙定炜,男,硕士,副研究员,主要从事病媒生物防制工作,E-mail:sdw_bmjc@163.com
  • 基金资助:
    海南省科技专项资助(ZDKJ2021035);海南省自然科学基金(821MS0844,821QN420)

Abstract: Objective To investigate the resistance of Anopheles sinensis to commonly used insecticides and the mutation of knockdown resistance (kdr) related gene in the voltage-gated sodium channel (VGSC) and acetylcholinesterase gene 1(ace-1 gene) encoding acetylcholinesterase in Sanya,Hainan province,China.Methods An.sinensis adults were captured in Tianya district,Sanya,Hainan province from September to October 2018 and September 2020.The resistance of field-captured An.sinensis to five commonly used insecticides was tested.Survival samples were collected from resistance testing,which were inspected for the mutation at the 1014 locus in the VGSC gene and the 119 locus in the ace-1 gene by the direct sequencing method.Results In 2018 and 2020,An.sinensis showed a resistance to deltamethrin (0.05%) and dichlorodiphenyltrichloroethane (DDT)(4.00%),with the 24 h mortality rates of 43.00% and 11.11% in 2018,and 71.00% and 39.18% in 2020,respectively.The resistance to malathion (5.0%) was changed from 91.00%(possible resistance) in 2018 to 100%(susceptible) in 2020.Sequencing results of polymerase chain reaction products showed that one mutation was detected at the 1014 locus in the VGSC gene and the 119 locus in the ace-1 gene,respectively,i.e.,L1014F leucine (L) mutated to phenylalanine (F)(TTG mutated to TTT) and G119S glycine (G) mutated to serine (S)(GGC mutated to AGC),and the mutation frequencies were 48.75% and 57.14% in 2018 and 52.50% and 43.75% in 2020,respectively.Conclusion An.sinensis from Sanya is resistant to deltamethrin and DDT but susceptible to malathion,etofenprox,and chlorfenapyr.The resistance-conferring mutation may lead to the resistance of An.sinensis to insecticides in Sanya,Hainan province.

Key words: Anopheles sinensis, Insecticides, Voltage-gated sodium channel gene, Acetylcholinesterase gene 1

摘要: 目的 了解海南省三亚市中华按蚊对常用杀虫剂的抗性状况及电压门控钠离子通道(VGSC)击倒抗性(kdr)相关基因及编码乙酰胆碱酯酶(AChE)的ace-1基因的突变情况。方法 分别于2018年9-10月和2020年9月在海南省三亚市天涯区采集中华按蚊成蚊,用生物测定法测定其对5种常用杀虫剂的抗药性。抽取抗性测定后存活样品,以直接测序法对VGSC基因1014位点和ace-1基因119位点的突变进行检测。结果 2018和2020年中华按蚊对0.05%溴氰菊酯和4.00%滴滴涕(DDT)均产生抗性,24 h死亡率分别为43.00%、11.11%和71.00%、39.18%;该蚊对5.0%马拉硫磷由2018年的可能抗性(91.00%)到2020年的敏感(100%)。PCR产物测序结果显示VGSC基因1014位点和ace-1基因119位点分别发现1种突变基因,即L1014F亮氨酸(L)突变为苯丙氨酸(F)(TTG突变为TTT)和G119S甘氨酸(G)突变为丝氨酸(S)(GGC突变为AGC),2018和2020年突变频率分别为48.75%、57.14%和52.50%、43.75%。结论 海南省三亚市中华按蚊对溴氰菊酯、DDT等产生抗性,而对马拉硫磷、醚菊酯、溴虫腈较敏感。抗性基因突变可能是导致中华按蚊产生抗药性的重要原因。

关键词: 中华按蚊, 杀虫剂, 电压门控钠离子通道基因, ace-1基因

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