Chinese Journal of Vector Biology and Control ›› 2022, Vol. 33 ›› Issue (5): 648-654.DOI: 10.11853/j.issn.1003.8280.2022.05.007

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Repellent efficacy of allicin long-acting repellents at different concentrations against larvae of Haemaphysalis longicornis

ZHAO Cong1, ZHANG Wen-ju2, NING Xiao-dong3, HUANG Bin-bin2, JIAN Yi-chen1, YUE Zhi-guang2, JIAN Fu-chun1, ZHANG Long-xian1, ZHANG Su-mei1, NING Chang-shen1   

  1. 1. Engineering College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, Henan 450002, China;
    2. Henan Anjin Biotechnology Co., Ltd., Zhengzhou, Henan 450003, China;
    3. Henan Vocational College of Applied Technology, Zhengzhou, Henan 450042, China
  • Received:2022-08-30 Online:2022-10-20 Published:2022-10-14

不同浓度大蒜素长效驱避剂对长角血蜱幼蜱的驱避效果研究

赵聪1, 张文举2, 宁晓冬3, 黄斌斌2, 菅忆晨1, 岳治光2, 菅复春1, 张龙现1, 张素梅1, 宁长申1   

  1. 1. 河南农业大学动物医学院, 河南郑州 450002;
    2. 河南安进生物技术股份有限公司, 河南 郑州 450003;
    3. 河南应用技术职业学院, 河南 郑州 450042
  • 通讯作者: 宁长申,E-mail:nnl1986@163.com
  • 作者简介:赵聪,男,硕士,从事动物寄生虫病防控工作,E-mail:1519953894@qq.com

Abstract: Objective To study the repellent effect of allicin on larvae of Haemaphysalis longicornis and the duration of action of allicin long-acting repellent.Methods The repellent effect of allicin solution (0.5,1.0,1.5,2.0,3.0,and 4.0 mg/ml) on larvae of H. longicornis was studied using an in vitro filter paper repellent device,and the repellent concentration 50(RC50) and repellent concentration 100(RC100) were calculated.The Kruskal-Wallis H test was used to compare the repellent effect every 48 h between different concentrations and dosage forms of allicin and three commercially available repellents (1% repellent ester long-lasting repellent,OFF repellent liquid,and Liushen Florida Water) and to determine its duration of action.Results When the concentration of allicin ranged from 0.5 mg/ml to 4.0 mg/ml,the repellent rate of allicin to larvae of H. longicornis increased gradually with the increase in concentration,and reached the maximum (100%) at 4.0 mg/ml;the RC50 and RC100 were 1.79 mg/ml and 3.52 mg/ml,respectively.The repellent efficacy of 0.5%,1.0%,and 3.0% allicin long-acting repellents was significantly higher than that of the respective water emulsion treatment group within 10 days (all P<0.05).Compared with the commercially available repellents,1.0% and 3.0% allicin long-acting repellents showed a better repellent activity against larvae of H. longicornis within 12 days.Conclusion 0.5%,1.0%,and 3.0% allicin long-acting repellents have the longest duration of action of 12,14,and 16 days,respectively and the effective protection duration of 2,4,and 6 days,respectively,and their repellent effect is better than that of commercially available repellents.

Key words: Allicin, Repellent test, Repellent, Haemaphysalis longicornis, Larva

摘要: 目的 研究大蒜素对长角血蜱幼蜱的驱避效果及其长效驱避剂的作用时间。方法 以长角血蜱幼蜱为测试对象,利用滤纸片体外驱避装置比较研究0.5、1.0、1.5、2.0、3.0、4.0 mg/ml 6种浓度大蒜素溶液对长角血蜱幼蜱的驱避效果,并计算半数驱避浓度(RC50)、全数驱避浓度(RC100);同时使用Kruskal-Wallis H检验研究不同浓度不同剂型大蒜素与3种市售驱避剂(1%驱蚊酯长效驱避剂、OFF驱蚊液、六神驱蚊花露水)每48 h的驱避效果,确定其作用时间。结果 大蒜素浓度在0.5~4.0 mg/ml内,随着浓度的增加,其对长角血蜱幼蜱的驱避率也逐渐上升,在4.0 mg/ml时达到最大,为100%,RC50及RC100分别为1.79和3.52 mg/ml;0.5%、1.0%、3.0%大蒜素长效驱避剂的驱避活性在10 d内显著大于各自浓度水乳液处理组(均P<0.05),与市售驱避剂相比,1.0%、3.0%大蒜素长效驱避剂在12 d内对长角血蜱幼蜱的驱避活性更好。结论 0.5%、1.0%、3.0%大蒜素长效驱避剂的最长作用时间分别为12、14和16 d,有效保护时间分别为2、4和6 d,驱避效果优于市售驱避剂。

关键词: 大蒜素, 驱避试验, 驱避剂, 长角血蜱, 幼蜱

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