Abstract: Objective To develop the method for ticks to feed on mouse blood under laboratory conditions.Methods The blood feeding chamber for ticks was prepared with commercially available materials.The attachment,engorgement,growth,and reproduction of Haemaphysalis longicornis were evaluated after feeding on mouse blood.Results The chambers were used to feed H.longicornis.Nymphs used 1-4 d to attach themselves to mice and had a mean biting success rate of 80.00%,an engorgement duration of 4-6 d and a mean engorgement rate of 98.21%.On the average,97.85% nymphs molted to adults.Adult ticks spent 6.5 d (M) to engorge with an engorgement rate of 66.67%,and the body weight of engorged ticks increased>150-fold versus unfed ticks.Each female tick laid an average of 876 eggs.On the average,78.90% eggs hatched with the duration of 25 d (M).Conclusion The blood feeding chamber for ticks developed by the authors is suitable for rearing H.longicornis under laboratory conditions.

Key words: Tick, Mouse, Blood feeding chamber, Rearing method, Life cycle

摘要： 目的 研发蜱实验室吸食小鼠血的方法。方法 使用市售材料制备蜱吸血小室，评价吸食鼠血后的长角血蜱附着时长、饱血、生长和繁殖情况。结果 通过蜱吸血小室供长角血蜱吸血，若蜱平均叮咬成功率为80.00%，叮咬时长1～4 d，吸血期4～6 d，平均饱血率为98.21%，蜕皮率为97.85%。成蜱饱血率为66.67%，吸血期中位数为6.5 d，饱血体质量比饥饿蜱增加了150多倍，每只雌蜱平均产卵数876粒，卵平均孵化率为78.90%，孵化期中位数为25 d。结论 研制的蜱吸血小室可用于长角血蜱实验室饲养。

关键词: 蜱, 小鼠, 吸血小室, 饲养方法, 生活史