Abstract:

Objective To analyze the pulsed-field gel electrophoresis (PFGE) band patterns of pathogenic Yersinia enterocolitica from live pigs in Chinese abattoirs and to provide a basis for the control of Y. enterocolitica. Methods Pharyngeal swabs (tonsils) and intestinal contents (at the ileocecal junction) were collected from the pigs in abattoirs in 11 provincial regions of China; Y. enterocolitica was isolated from the obtained specimens, and the pathogenic strains were analyzed by PFGE. Results The pathogenic strains of Y. enterocolitica were subjected to PFGE typing. The 763 pathogenic strains of Y. enterocolitica serotype O:3 were classified into 47 patterns after cleavage with NotⅠ, and 431 (56.49%) of the 763 strains had the band pattern of K6GN11C30021; another serotype O:9 strain had the band pattern of K6GN11C90004. Two or more PFGE patterns were found in each abattoir, including dominant and common PFGE patterns. Conclusion There are diverse PFGE patterns of pathogenic Y. enterocolitica among the live pigs in Chinese abattoirs, with some regional characteristics, and the Y. enterocolitica with different PFGE patterns can be simultaneously carried in the pharyngeal region and the intestinal contents at the ileocecal junction. Measures should be taken to prevent Y. enterocolitica carried by pigs from contaminating pork and its products and polluting the environment and to protect public health and ensure food safety.

Key words: Yersinia enterocolitica, Pig, Pulsed-field gel electrophoresis

摘要：

目的 对中国屠宰场生猪中致病性小肠结肠炎耶尔森菌脉冲场凝胶电泳(PFGE)带型分布进行分析,为该菌防控提供依据。方法 从11个省(直辖市、自治区)屠宰场采集猪咽拭子和猪回盲部肠内容物标本分离小肠结肠炎耶尔森菌,同时对致病性菌株进行PFGE分析。结果 采用PFGE分型用NotⅠ酶切后将763株致病性O:3血清型菌株分为47个型别,其中K6GN11C30021带型是猪中携带的致病菌株优势PFGE 型别,占56.49%(431/763); 另外1株O:9血清型菌株为K6GN11C90004型别。所有的屠宰场皆有多种PFGE 带型,不同地区除具有各自的优势型别外存在交叉带型。结论 中国屠宰场生猪中致病性小肠结肠炎耶尔森菌PFGE带型多样,具有一定地域性特征。且猪咽部与回盲部肠内容物中能同时携带多种带型的小肠结肠炎耶尔森菌。应警惕猪中携带的小肠结肠炎耶尔森菌通过污染猪肉及其制品以及环境而感染人类,从而对公共卫生和食品安全构成威胁。

关键词: 小肠结肠炎耶尔森菌, 猪, 脉冲场凝胶电泳