Chines Journal of Vector Biology and Control ›› 2012, Vol. 23 ›› Issue (4): 292-294.

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Molecular epidemiological investigation on hantavirus carried by rodents in Fujian port

WANG Yu-ping1, XU Qing1, CAI Heng-zhong1, CHEN Jun2, ZHANG Jian-ming1, ZHENG Yan-ping1, GAO Bo1   

  1. 1 Fujian International Travel Healthcare Center, Fuzhou 350001, Fujian Province, China;
    2 Public Health School of Fujian Medical University
  • Received:2012-04-15 Online:2012-08-20 Published:2012-08-20
  • Supported by:
    Supported by General Administration of Quality Supervision, Inspection and Quarantine(No. 2010IK217)and Fujian Entry-Exit Inspection and Quarantine Bureau(No. FK2010-15)

福建口岸啮齿类动物携带汉坦病毒分子流行病学调查

王宇平1, 许卿1, 蔡亨忠1, 陈钧2, 张建明1, 郑燕平1, 高博1   

  1. 1 福建国际旅行卫生保健中心, 福建福州 350001;
    2 福建医科大学
  • 基金资助:
    国家质检总局科研基金项目(2010IK217);福建检验检疫局科研基金项目(FK2010-15)

Abstract: Objective To identify the genotype and analyze molecule characteristics of main Hantavirus in Fujian port. Methods Nested RT-PCR was used to detect Hantavirus-specific nucleic acid sequences in rodents captured in Fujian port, and M segment was sequenced and analyzed in positive samples. Results A total of 839 rodents were trapped in Fujian port in 2010. Rattus norvegicus was the dominant species accounting for 58.52%. The positive rate of Hantavirus in captured rodents was 5.84%, which were all of Seoul Virus (SEOV). Hantavirus positive species were mainly R. norvegicus (8.96%) and R. tanezumi (3.09%). Generally, Hantavirus infection significantly increased during winter and spring. The phylogenetic analysis based on partial M segment (nt2001-2301) sequence in 25 samples indicated that there were two genetic subtypes, Seoul 2 and Seoul 3. Conclusion Seoul 3 is the main hantavirus subtype carried by rodents in Fujian port.

Key words: Rodent, Hantavirus, Genotype, Phylogenetic tree

摘要: 目的 了解福建口岸汉坦病毒的基因型别和分子特征。方法 对2010年福建口岸捕获的鼠形动物进行汉坦病毒检测,对部分阳性标本进行M基因片段序列分析。结果 2010年福建口岸共捕获鼠形动物839只,以褐家鼠为优势鼠种,占58.52%;鼠形动物携带汉坦病毒阳性率5.84%,均为汉城型(SEO),阳性鼠种为褐家鼠和黄胸鼠,阳性率分别为8.96%和3.09%。汉坦病毒检出呈较明显的冬春季高峰。用M基因片段(nt2001~2301)核苷酸序列构建系统进化树,将福建口岸SEOV分为S2和S3两个亚型。结论 S3亚型SEOV为福建口岸优势流行亚型。

关键词: 啮齿类动物, 汉坦病毒, 基因型, 系统进化树

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