ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chines Journal of Vector Biology and Control
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XU Fang; YAO Ping-ping; ZHU Han-ping; XIE Rong-hui
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徐芳;姚苹苹;朱函坪; 谢荣辉
Abstract: Objective To investigate the epidemiology of the hemorrhagic fever with renal syndrome(HFRS) and the serotype of hantavirus(HV).Methods Direct immunoflorscence assay(DFA) was adopted to detect HFRS antigen and the virus was isolated from the young Meriones unguiculata inoculated with the rat lung tissues infected by HV.The isolated virus were continuously propagated in the Vero-E6 cell and identified by DFA using Anti-HV McAb.Results Three strains of HV were isolated from the eight lung tissues infected by HV which belonged to the hantaan type.The virus titer of three strains could reach 104.75-105.25 CCID50/ml in the Vero-E6 cell.Conclusion Three strains of hantaan virus were successfully isolated from the lung tissue of rats,which could be the candidate strain for HFRS vaccine.
摘要: 目的 了解浙江省肾综合征出血热(HFRS)流行特点及汉坦病毒(HV)型别,为防治提供科学依据。方法 鼠肺HV抗原检测采用直接免疫荧光法(DFA),病毒分离采用阳性鼠肺研磨液接种幼龄长爪沙鼠,再经Vero-E6细胞传代,用单克隆抗体DFA鉴定毒株及分型。结果 从捕自浙江省龙泉地区的8份阳性鼠肺中分离到3株HV,经DFA鉴定均为汉滩型(Ⅰ型)病毒,3株病毒在Vero-E6细胞中的感染滴度为104.75~10M5.25CCID50/ml。结论 从鼠类肺组织中分离到3株汉滩型HV,毒株均能在Vero-E6细胞中较好增殖,有可能成为HFRS疫苗后备毒株。
关键词: 汉坦病毒, 汉滩型, 啮齿动物
XU Fang; YAO Ping-ping; ZHU Han-ping; XIE Rong-hui. Isolation and identification of 3 strains of hemorrhagic fever with renal syndrome virus[J]. Chines Journal of Vector Biology and Control.
徐芳;姚苹苹;朱函坪; 谢荣辉. 3株肾综合征出血热病毒的分离和鉴定[J]. 中国媒介生物学及控制杂志.
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