ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chines Journal of Vector Biology and Control
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JIANG Li-ping; ZHENG Shou-gui; MO Shi-hua; YE Xiao-dong; ZHAN Li; WANG Zhi-gang
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姜理平1;郑寿贵2;莫世华1; 叶晓东2; 占利1; 王志刚1
Abstract: Objective To investigate the natural infection situation of Borrelia burgdorferi sensu lato in rat-shape animals from Zhejiang province.Methods The segment between 5S rRNA and 23S rRNA of B.burgdorferi sensu lato in the rat-shape animals was amplified with the method of nest-PCR,and the positive PCR products were sequenced and analyzed.Results 128 rat-shape animals were captured in Jindong district of Jinhua city.12 positive samples were detected in Rattus,Apodemus and Shrew,and the positive rate were 9.68%,14.28% and 25.00%,respectively.The sequence analysis results showed the amplified segments from two positive samples were homologous to that of B.valaisiana and their gene serial numbers were AB091447BvalaiOS115 and DQ188933BvalaiQX13P25.The comparability reached 100% between them.Conclusion It suggested that there was B.burgdorferi sensu lato in Zhejiang province,and the rice field mouse,Apodemus and shrew could be the hosts of B.valaisiana.But it still needs be further studied.
摘要: 目的 了解鼠形动物中自然感染伯氏疏螺旋体的状况。方法 用巢式聚合酶链反应扩增鼠中的伯氏疏螺旋体5S~23S rRNA间隔区片段,对阳性产物进行测序。结果 在浙江省金华市金东区捕获鼠形动物128只,在黄毛鼠、姬鼠、臭鼩鼱(鼠青)中共检测到阳性12份,阳性率分别为9.68%、14.28%和25.00%,并对其中2份进行测序分析,与伯氏疏螺旋体的法雷斯疏螺旋体相同,基因区间序列号分别为AB091447BvalaiOS115和DQ188933BvalaiQX13P25,相似率达100%。结论 初步认为浙江省存在伯氏疏螺旋体病原。黄毛鼠、姬鼠、臭(鼠青)可能为伯氏疏螺旋体的法雷斯疏螺旋体宿主,尚需进一步调查。
关键词: 鼠类, 伯氏疏螺旋体, 聚合酶链反应
JIANG Li-ping; ZHENG Shou-gui; MO Shi-hua; YE Xiao-dong; ZHAN Li; WANG Zhi-gang. Detection and sequence analysis of Borrelia burgdorferi sensu lato DNA in rat-shape animals from Zhejiang province[J]. Chines Journal of Vector Biology and Control.
姜理平1;郑寿贵2;莫世华1; 叶晓东2; 占利1; 王志刚1. 浙江省在鼠形动物中检测到伯氏疏螺旋体[J]. 中国媒介生物学及控制杂志.
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