ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chines Journal of Vector Biology and Control
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LIU Hui; YAN Rong; HE Li-fang; WAN Qi-hui
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刘晖;晏容;贺莉芳; 万启惠
Abstract: Objective The hemocytes of the 3rd instar larva of Musca domestica were observed and classified with fluorescent staining methods and phase contrast microscope. Methods The hemocytes of the 3rd instar larva of M.domestica were stained by acridine orange(AO) and propidium iodide(PI) and the morphologic characteristics were observed and classified. Results (1) The hemocytes of the 3rd instar larvae of M.domestica were divided into prohemocyte, plasmatocyte, granulocyte, sphrulocyte and oenocytoid. The plasmatocytes were divided into big nucleus hematocyte and micronucleus hematocyte. (2) The prohemocyte was easily identified with phase contrast microscope, but its character was not notable by fluorescent staining methods. (3) The granulocyte and big nucleus plasmatocyte, sphrulocyte and micronucleus plasmatocyte were confusable with phase contrast microscope, but they were easily distinguished with fluorescent microscope. Conclusion The fluorescent staining methods combined with phase contrast microscope can be used to observe unknown morphologic characterstics of the insect hemocytes, and the hemocytes of the 3rd instar larva of M.domestica could be accurately divided into five types.
摘要: 目的 用荧光染色法结合相差显微镜观察家蝇3龄幼虫血细胞的形态、分类。方法 应用吖啶橙和碘化丙啶对家蝇3龄幼虫血细胞进行染色,荧光显微镜结合相差显微镜观察血细胞形态特征并进行分类。结果 (1)家蝇3龄幼虫血细胞可分为原血胞、浆血胞、粒血胞、珠血胞、类绛血胞5类。其中浆血胞又分为大核浆血胞和小核浆血胞2种。(2)家蝇幼虫原血胞在相差显微镜下容易辨认,通过荧光染色后特征不显著。(3)家蝇幼虫粒血胞与大核浆血胞、珠血胞与小核浆血胞在相差显微镜下容易混淆,但在荧光显微镜下很好区分。结论 用荧光染色法结合相差显微镜能揭示未知的昆虫血细胞特征,并能正确地将家蝇3龄幼虫血细胞分为5类。
关键词: 家蝇幼虫, 血细胞, 形态学, 吖啶橙, 碘化丙啶
LIU Hui; YAN Rong; HE Li-fang; WAN Qi-hui. Observation on hemocytes morphology of the Musca domestica larva stained by fluorescent staining methods[J]. Chines Journal of Vector Biology and Control.
刘晖;晏容;贺莉芳; 万启惠. 家蝇幼虫血细胞荧光染色法的形态观察[J]. 中国媒介生物学及控制杂志.
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