Abstract: Objective To establish a sensitive and specific method for detecting total antibodies in sera of hemorrhagic fever with renal syndrome(HFRS) patients and rodents by the double antigens sandwich ELISA. Methods The double antigens sandwich ELISA was established with coating antigen e1.3S from Hantavirus (HV), combining with recombinant antigen e6-119 labeled with HRP. The method was used to detect total antibodies in sera of HFRS patients and rodents, and the results were compared to that of indirect fluorescent assay(IFA). Results Five hundred and sixty-six samples (188 human sera and 378 rodents sera) were detected by using the double antigens sandwich ELISA and IFA. The total coincidence rate of the results by the two methods was 97.70%. Compared with IFA, the ELISA for the detection of total antibodies showed 97.54% in sensitivity and 97.87% in specificity. Conclusion The double antigens sandwich ELISA showed higher sensitivity and specificity for the detection of total antibodies, which can be applied in the large-scale epidemiologic survey.

摘要： 目的 建立一种敏感、特异的检测肾综合征出血热(HFRS)血清总抗体的双抗原夹心法ELISA。方法 应用重组汉坦病毒(HV)NP抗原e1.3S作为包被抗原和e6-119-HRP作为酶标抗原建立双抗原夹心法ELISA,检测血清总抗体,并与间接免疫荧光法(IFA)相比较。结果 共检测188份人血清和378份鼠血清标本,2种方法的总符合率为97.70%。以IFA为参照标准,双抗原夹心法ELISA的敏感性为97.54%,特异性为97.87%。结论 双抗原夹心法ELISA检测HFRS血清总抗体具有很高的敏感性和特异性,适用于大规模的流行病学调查。

关键词: 肾综合征出血热, 双抗原夹心法ELISA, 血清学诊断