Abstract: Objective Synthesize Aedes aegypti larvae gut cDNA for library construction.Methods Feed Ae.aegypti,collect larvae gut,isolate RNA and mRNA,synthesize cDNA.Results 6 μg larvae gut cDNA of Ae.aegypti were successfully synthesized,A260/A₂₈₀ is 1.95,DNA fragment size is larger than 400 bp.Conclusion Ae.aegypti larvae gut cDNA was synthesized in good quality with FastTrack Kit and Copy Kit of Invitrogen Life technologies.This may be a good foundation for Ae.aegypti larvae gut cDNA library construction.

摘要： 目的 合成埃及伊蚊蚊幼肠道cDNA,为进一步构建伊蚊蚊幼肠道cDNA基因文库奠定基础。方法 饲养埃及伊蚊,收集蚊幼肠道并提取mRNA,合成cDNA。结果 成功地合成了埃及伊蚊蚊幼肠道cDNA 6μg,纯度达到A₂₆₀/A₂₈₀1.95,片段大于400 bp。结论 使用Invitrogen Life technologies公司的FastTrack试剂盒和Copy Kit试剂盒成功地合成了高质量的cDNA,为伊蚊蚊幼肠道cDNA基因文库构建奠定了基础。

关键词: 埃及伊蚊, 蚊幼, cDNA合成, 肠道