ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chines Journal of Vector Biology and Control
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YI Jian-rong; LIN Li-feng;DUAN Jin-hua; et al
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易建荣;林立丰;段金花;吴军;裴福全;卢文成;蔡松武;郑焕英;阴伟雄
Abstract: Objective To investigate SARS virus carried by murine-like animals in the hygienic units to provide evidence for looking for the origin and infectious ways of SARS virus.Methods Rats were caught in usual ways.Nested RT-PCR,Fluorescent quantitative PCR,Sequence analysis and virus isolation were applied.Results There are 59 murine-like animals of three species caught in the hygienic units related to SARS in Guangzhou.32 samples of anus swabs test were tested in nested RT-PCR.The resnlts showed that four samples were positive.The positive rate was 12.5 percent.The sequence of positive parts had 90%~96% homogeneity with SARS-CoV.The result of the cell cultivation on positive samples of PCR amplification parts was negative.Conclusion It would be studied further to conform whether murine-like animals can carry and transmit SARS coronavirus.
摘要: 目的调查与SARS相关的卫生医疗机构内鼠形动物携带SARS冠状病毒(CoV)情况,为寻找SARS的源头和传染途径提供证据。方法常规方法捕捉老鼠,采用巢式PCR技术、荧光定量PCR技术、基因序列检定技术、病毒细胞培养技术进行测试。结果在广州市与SARS相关的卫生医疗机构内捕捉的鼠形动物共3种59只;用SARSCoV特异性引物对32份肛拭子样本进行PCR检测,结果显示肛拭子样本有4份阳性,阳性率为12.5%;阳性片段基因序列与SARSCoV同源性为90%~96%;PCR扩增片段阳性样本细胞培养结果未能分离到SARSCoV。结论鼠形动物能否携带和传播SARSCoV,仍需作进一步研究。
关键词: SARS冠状病毒, 鼠形动物, 聚合酶链反应, 序列分析, 病毒分离
YI Jian-rong; LIN Li-feng;DUAN Jin-hua; et al. Analysison Murine-like Animals Carrying SARSC or onavirusin the Hygienic Units Related to SARS[J]. Chines Journal of Vector Biology and Control.
易建荣;林立丰;段金花;吴军;裴福全;卢文成;蔡松武;郑焕英;阴伟雄. 与SARS相关的卫生医疗机构内鼠形动物携带SARS冠状病毒的调查分析[J]. 中国媒介生物学及控制杂志.
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