Abstract: Objective To construct an expression vector carrying the outer surface protein C gene of Borrelia burgdorferi which was cloned and expressed for studies in prevention,diagnosis and pathogenic mechanism of Lyme disease.Methods The ospC gene was amplified from the genome of Borrelia burgdorferi PD₉₁ strain by PCR and recombined with plasmid PET-11D.The recombinant plasmid PET- 11D-ospC was identified with restriction endonuclease analysis and sequencing.Results The ospC gene was cloned correctly into vector PET-11D.The homology of nucleotide sequence of the inserted fragment in the plasmid was between 62% to 86% compared with the published sequence of ospC gene.Conclusion The homology of PD₉₁ strain presented highly difference with foreign isolates,and the PET- 11D-ospC constructed successfully pave the way for the research of Lyme disease.

摘要： 目的构建伯氏疏螺旋体PD₉₁菌株外膜蛋白C(OspC)的表达载体,克隆表达OspC,用于莱姆病的预防、诊断和致病机理上的研究。方法用PCR扩增PD₉₁ospC基因,定向克隆到表达载体PET-11D,构建重组质粒。采用酶切分析及序列测定等方法鉴定重组质粒的正确性。结果ospC基因被正确克隆到表达载体PET-11D中。序列测定结果证实与已报道的ospC基因序列同源性介于62%～86%之间。结论我国PD₉₁菌株的ospC编码基因与已报道菌株的ospC菌株在同源性上存在较大的差异。PET-11D-ospC重组质粒的成功构建为我国莱姆病的进一步研究奠定了基础。

关键词: 伯氏疏螺旋体, 外膜蛋白C, 克隆, 同源性