ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chines Journal of Vector Biology and Control
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GUO Xiao-xia; ZHAO Tong-yan; DONG Yan-de;et al
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郭晓霞1;赵彤言1;董言德1;蒋书楠2;陆宝麟1
Abstract: Objective To determine whether DEN-2 can survive in diapause eggs and be transmitted vertically to progeny of infected Aedes albopictus.Methods Reverse transcription-polymerase chain reaction(RT-PCR) combined with virus isolation in C6/36 cell were used for assay of the DEN-2 in diapause eggs and progeny of infected Aedes albopictus in labortary.Results DEN-2 was detected in diapause eggs and progeny after termintaed diapause.The total positive rate of pools was 9.6%,the minimum infection rate of the progeny was 1:294.3;DEN-2 was not isolated from the progeny of the first oviposition cycle.The minimum infection rates were not significantly different for the second and third oviposition cycle(χ2=0.01,P>0.05).Conclusion DEN-2 can survive in diapause eggs and be transmitted vertically to progeny of Aedes albopictus.
摘要: 目的证明登革Ⅱ型病毒能否在白纹伊蚊滞育卵内存活并传至子代。方法采用逆转录-聚合酶链反应检测病毒,C636细胞培养分离病毒。结果能在白纹伊蚊滞育卵内检测到病毒,且滞育卵解除滞育后,子1代幼虫和成蚊中均检测到病毒,总的批阳性率为9.6%,子代最低感染率为1∶294.3;第一个生殖营养周环子代未分离到病毒;第二、三生殖营养周环子代间最低感染率差异无显著性(χ2=0.01,P>0.05)。结论试验证明登革Ⅱ型病毒能在白纹伊蚊滞育卵内存活并传至子代。
关键词: 白纹伊蚊, 登革Ⅱ型病毒, 垂直传递, 滞育卵
GUO Xiao-xia; ZHAO Tong-yan; DONG Yan-de;et al. Study on Diapause Eggs of Aedes albopictus Vertical Transmission of DEN-2[J]. Chines Journal of Vector Biology and Control.
郭晓霞1;赵彤言1;董言德1;蒋书楠2;陆宝麟1. 白纹伊蚊经滞育卵传递登革Ⅱ型病毒的实验研究[J]. 中国媒介生物学及控制杂志.
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