ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chines Journal of Vector Biology and Control
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WEI Jian-chun; HAI Rong; ZHANG Zhi-kai; et al
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魏建春;海荣;张志凯;俞东征
Abstract: Objective:To develop a kind of method for detection of Bacillus Anthracis in the environment.Methods:Plasmid extraction and PCR amplification.Result:2 pairs of primers were used,and 2 different fragments were obtained from the positive samples.Conclusion:The PCR amplification can be as a method for detection of the Bacillus anthracis in the environment,moveover,this method can evaluate pathogenicity of the Bacillus anthracis.
摘要: 目的:快速检测炭疽芽孢杆菌。方法:质粒提取及PCR扩增。结果:用两对引物扩增,阳性样本可分别扩出两条877bp和1089bp左右的条带,阴性样本未扩增出。结论:所采用的方法可快速检测出环境中的炭疽芽孢杆菌,并可对其致病性进行评价。
关键词: 炭疽芽孢杆菌, 聚合酶链式反应
WEI Jian-chun; HAI Rong; ZHANG Zhi-kai; et al. Detection of Bacillus Anthracis in the Environment Using Rapid PCR[J]. Chines Journal of Vector Biology and Control.
魏建春;海荣;张志凯;俞东征. PCR方法快速检测炭疽芽孢杆菌[J]. 中国媒介生物学及控制杂志.
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