ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chines Journal of Vector Biology and Control
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ZHU Li-hua; ZHAO Tong-yan; LIU Xian-kai; et al
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朱礼华;赵彤言;刘先凯;董言德;陆宝麟
Abstract: Objective:To study the factor Xagene-directed anti-coagulant(AFXA) from Aedes aegypti in China in molecular biologic levels.Methods:Degenerate oligo-nucleotide primers PCR was used to amplify from its genome DNA,then the amplified fragment was cloned into T vector to sequence.Results:A 300bp AFXA gene fragments was obtained and sequenced.There was a 63bp intron in it comparing with cDNA sequence.Conclusion:This gene fragment from Haikou strain may be a new gene type of factor Xagene-directed anti-coagulant.
摘要: 目的:探讨我国登革热的重要媒介蚊种埃及伊蚊唾液中的抗凝血因子Xa基因。方法:用兼并引物PCR方法从我国的埃及伊蚊基因组DNA扩增抗凝血因子Xa基因片段,克隆入测序T载体进行测序。结果:扩增出抗凝血因子Xa基因片段长度约300bp,测序后发现与报道的埃及伊蚊的cDNA基因相应序列多了一段63bp内含子,并有几个碱基和氨基酸序列变化。结论:我国海南岛的埃及伊蚊抗凝血因子Xa基因有一定差异,可能对应的是一个新的基因型。
关键词: 伊蚊属, 埃及伊蚊, 抗凝血因子Xa, 聚合酶链反应
ZHU Li-hua; ZHAO Tong-yan; LIU Xian-kai; et al. Part Gene Sequence of Factor Xa-directed Anticoagulant from the Vector Mosqui to Aedes aegypti in China[J]. Chines Journal of Vector Biology and Control.
朱礼华;赵彤言;刘先凯;董言德;陆宝麟. 埃及伊蚊抗凝血因子Xa基因片段的克隆测序[J]. 中国媒介生物学及控制杂志.
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