Abstract: Objective:To establish a new method for gene detection of Brugia malayi larvae in mosquito and use it in filariasis surveillance.Methods:Oligonucleotide fragment of Brugia malayi synthesized by genetic engineering technique was labeled with ³²P and used as probe.The B.malayi larvae in mosquito was detected with dot blot.Results: Brugia malayi larvae DNA could be specifically detected in different samples with this probe and the sensitivity was 2 ng target DNA of B.malayi.Even only one larvae in mosquito could be detected after hybridization.Conclusions:This method is practicable and reliable for gene detection of Brugia malayi larvae in mosquito.

摘要： 目的:探索蚊体内马来丝虫幼虫基因检测的新方法,并用于丝虫病监测。方法:利用基因工程技术合成马来丝虫寡核苷酸片段,经³²P标记后作为探针,以斑点杂交法检测蚊体内马来丝虫幼虫。结果:该探针可从多种标本中特异地检出马来幼丝虫DNA,敏感性为2ng靶DNA量,蚊体内含1条幼虫即可出现阳性杂交。结论:该技术具有良好的实用性,可用于蚊体内马来丝虫幼虫的基因检测。

关键词: 基因检测, 探针, 马来幼丝虫