ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chines Journal of Vector Biology and Control
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Wang Li-li; Huo Xi-xiang
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王莉莉; 霍细香
Abstract: Application was conducted for detecting antibody levels of population in endemic area with Malayan filariasis(microfilarial rate being 0.08-0.72%) by using three serologic tests, IFAT,ELISA and Dot-ELISA in 1990, 1991 and 1994. The results showed that the positive rate were 24.58%, 20.32% and 8.3% with IFAT; 21.66%, 30.32% and 15.84% with ELISA; 32.5%,10.96% and 18.86% with Dot-ELISA; and the total positive rates were 46.66%, 35.8% and 32.07% respectively. The results showed that IFAT were found to be more stable. It was also proved that accumulative detection rate of using three tests be higher than each of the three tests.
摘要: 目的:比较三种方法监测马来丝虫病的效果。方法:间接荧光抗体试验(IFAT)、酶联免疫吸附试验(ELISA)、斑点-酶联吸附试验(Dot-ELISA)。结果:检测1990、1991、1994年基本消灭马来丝虫病的流行区人群血清,IFAT阳性率分别为24.85%、20.32%和8.30%;ELISA阳性率分别为21.66%、30.32%和15.84%;Dot-ELISA阳性率分别为32.50%、10.96%和18.86%;总阳性率分别为46.66%、35.80%和32.07%。结论:以IFAT法较为稳定,3种方法联合应用,可提高阳性率。
关键词: 马来丝虫病, 血清学监测, 间接荧光抗体试验, 酶联免疫吸附试验, 斑点-酶联免疫吸附试验
Wang Li-li; Huo Xi-xiang. Applications of Three Serologic Tests for Surveillanceof Malayan Filariasisin Endemic Area[J]. Chines Journal of Vector Biology and Control.
王莉莉; 霍细香. 三种血清学方法对马来丝虫病监测的应用研究[J]. 中国媒介生物学及控制杂志.
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