Abstract: From 1994 to 1995,the vector of Lyme disease spirochetea was investigated in the Small Xing-An Mountains of Heilongjiang province,China.Ixodes persulcatus of Ixodidae was prevailing in this region.Spirochetes were observed in midguts smear by direct immunofluorescence test.20 of 50 (40 percent) Ixodes persulcatus contained spirochetea.Two isolates were discovered from 14 group (70 Ixodes persulcatus) and these isolates were distinguished between China and USA by monoclonal antibodies (H5332,H6831 and H9724).These studies indicate that Ixodes persulcatus is primary vectors that transmit the spirochetes to human and other animals and this region is an endemic area of Lyme disease.

摘要： 目的：探讨全沟硬蜱在莱姆病螺旋体传播中的作用。方法：用布旗法在林区草地采蜱，并分类鉴定；用直接荧光抗体法检查蜱类中肠带菌情况，并分离菌株。结果：全沟硬蜱为当地优势蜱种，人群发病与该蜱叮咬率密切相关；该蜱中肠带菌率为40％，并从14组（70只）蜱中分离出2株莱姆病螺旋体；新分离株的单克隆抗体反应与国内分离株M₇相同，而与美国菌株B₃₁有区别。结论：当地全沟硬蜱是莱姆病螺旋体的主要生物媒介。

关键词: 莱姆病螺旋体, 全沟硬蜱