Abstract: A rapid fluorescent focus inhibition test of hemorrhagic fever with renal synidrom virus (HFRSV) has been established.All four HFRSV strains tested could form fluorescent focus on Vero-E cell culture.A linear re1ationshlp between the number of nuorescent focus and virus concentration was observed.By comparative testing of 20 samples of sera, it was proved that sensitivity of detecting neutralizing antibodies to HFRSV by RFFIT was sirnilar to that by PRNT.However, this method is easier, faster and more specific than the others.

摘要： 报道一种新的HFRS病毒中和抗体检测方法──快速荧光灶抑制试验的建立。方法：病毒和细胞同时加入塑料微孔板上，37℃培养24小时后，经固定和荧光染色，即可在荧光显微镜下判定结果。结果：检验的4株病毒能在Vero－E₆细胞上形成荧光灶；接种病毒的浓度与荧光灶里直线关系。用此法检测Z10单价和双价灭活疫苗免疫的20份兔及人血清的中和抗体，与空斑减少中和试验的结果相一致。结果：此法具有快速、简便、敏感、稳定的特点。

关键词: 肾综合征出血热病毒, 快速荧光灶抑制试验