ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chines Journal of Vector Biology and Control
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Zhang Yong-Hua; Chen Hua-xin
Online:
Published:
张永华; 陈化新
Abstract: Six hybridoma cell lines producing monoclonal antitalies to hantavir-us were established by fusion of sp2/0 mice rnyeloma cells with spleen cells of BALB/c mice immunized with Hantavirus strain 76- 118 and R22.Among these, two were of the IgGl, one was of IgG2b and three were of IgG2a.Ascitic titres of the antibodies ranged from 1: 10 240 to 1: 81 920 when tested by indirect immunofiuorescent antibody staining rnethod.Reversed passive hemagglutination inhibition test dernonstrated that the hemagglutination inhibition titres of the six antibodies were lower than l: 20.Western-blotting assay showed the six antitheies were all binding to the nucleocapsid protein.According to cross-reactivities with other hantavirus, the antibodies were classified into three groups: 3 antitheies cross-reacting with Hantaan and Seoul virus strains, l specific to Hantaan serotype and 2 specific to seul serotyPe.6 antibodies were also used in antigenic analysis of virus strains isolated from Ningxia.
摘要: 本文用汉坦病毒A16和R22株活毒免疫BALB/c小鼠,取牌细胞与sp2/0骨髓瘤细胞融合,制备了6株分泌抗汉坦病毒单克隆抗体的杂交癌细胞系。单克隆抗体IgG亚类鉴定表明,其中2株为IgG1,1株为IgG2b,3株为IgG2a。用间接免疫荧光检测单抗腹水滴度,分别在1:10240~1:81920之间;用反向被动血凝抑制试验测定单抗腹水,滴度均低于1:20。免疫印迹试验表明,6株单克隆抗体均为抗核蛋白单抗。对已知型别病毒的反应话表明,其中3株为组特异性单抗,1株为汉滩型特异性单抗,2株为汉城型特异性单抗。还用6株单抗对宁夏新分离毒株进行抗原性分析。
关键词: 单克隆抗体, 汉坦病毒
Zhang Yong-Hua; Chen Hua-xin. Preparation and Characterization of Monoclonal Antibodies against Hantavirus[J]. Chines Journal of Vector Biology and Control.
张永华; 陈化新. 汉坦病毒单克隆抗体的制备和鉴定[J]. 中国媒介生物学及控制杂志.
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