ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chines Journal of Vector Biology and Control
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Lan Ming-yang;et al
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蓝明扬1; 沈伟华1; 黄超群1; 蒋滢1; 顾萱娣1; 于心2; 叶瑞玉2; 赵郁光3; 陆卫贤3
Abstract: We reported the study on polymorphism DNA of Boophilus microplus with RAPD-PCR technique in the first instance.Here,we amplified the genomic polymorphism DNA maps of Hyaloma asiaticum asiaticum with three primers-“Pz” (CGGCCCGGTA),“Pw”(CGGCCCCGGT) and“Py”(CTGGAGGACT).The molecular weights (bp) of the DNA bands for each map were:“Pz”: 1380,1230,955,912,871,794,741,668,646,562, 537,442; "Pw":955,724,668,562,537,457,279; "Py":1072,912,832,794,612,582,562,537,422,385. The RAPD-PCR for its simplicity, rapidness,and being able to reveal difference and evolutional relationships at the level of DNA,could be developed into an ideal taxonomic method for Acarology.
摘要: 我们首先报道了RAPD-PcR技术研究微小牛蜱DNA的多态性。本文则用3个引物"Pz"(CGGCCCGGTA)、"Pw"(CGGCCCCGGT)和"Py"(CTGCAGGACA)对亚洲璃眼蜱基因组DNA作RAPD-PCR,均能扩增出多态性DNA图谱;各引物扩增的DNA片段的分子大小是:"PZ"为1380、1230、955、912、871、794、741、668、646、562、537、442;"PW"为955、724、668、562、537、457、279;"Py"为1072、912、832、794、612、582、562、537、422、385。RAPD-PCR技术简便、快速,并能在DNA水平上揭示其差异和进化关系,可发展成为理想的蜱类分类学技术之一。
关键词: 亚洲璃眼蜱, 基因组, 多态DNA聚合酶链技术
Lan Ming-yang;et al. Study on the Genomic Polymorphism DNA of Hyaloma asiaticum asiaticum[J]. Chines Journal of Vector Biology and Control.
蓝明扬1; 沈伟华1; 黄超群1; 蒋滢1; 顾萱娣1; 于心2; 叶瑞玉2; 赵郁光3; 陆卫贤3. 亚洲璃眼蜱基因组多态DNA的研究[J]. 中国媒介生物学及控制杂志.
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